2ger: Difference between revisions

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Crystal Structure and Oxidative Mechanism of Human Pyrroline-5-carboxylate Reductase

OverviewOverview

Pyrroline-5-carboxylate reductase (P5CR) is a universal housekeeping enzyme that catalyzes the reduction of Delta(1)-pyrroline-5-carboxylate (P5C) to proline using NAD(P)H as the cofactor. The enzymatic cycle between P5C and proline is very important for the regulation of amino acid metabolism, intracellular redox potential, and apoptosis. Here, we present the 2.8 Angstroms resolution structure of the P5CR apo enzyme, its 3.1 Angstroms resolution ternary complex with NAD(P)H and substrate-analog. The refined structures demonstrate a decameric architecture with five homodimer subunits and ten catalytic sites arranged around a peripheral circular groove. Mutagenesis and kinetic studies reveal the pivotal roles of the dinucleotide-binding Rossmann motif and residue Glu221 in the human enzyme. Human P5CR is thermostable and the crystals were grown at 37 degrees C. The enzyme is implicated in oxidation of the anti-tumor drug thioproline.

About this StructureAbout this Structure

2GER is a Single protein structure of sequence from Homo sapiens. Active as Pyrroline-5-carboxylate reductase, with EC number 1.5.1.2 Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of human pyrroline-5-carboxylate reductase., Meng Z, Lou Z, Liu Z, Li M, Zhao X, Bartlam M, Rao Z, J Mol Biol. 2006 Jun 23;359(5):1364-77. Epub 2006 May 11. PMID:16730026

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OCA

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 '''Crystal Structure and Oxidative Mechanism of Human Pyrroline-5-carboxylate Reductase'''<br />
 ==Overview==
-Pyrroline-5-carboxylate reductase (P5CR) is a universal housekeeping, enzyme that catalyzes the reduction of Delta(1)-pyrroline-5-carboxylate, (P5C) to proline using NAD(P)H as the cofactor. The enzymatic cycle, between P5C and proline is very important for the regulation of amino acid, metabolism, intracellular redox potential, and apoptosis. Here, we present, the 2.8 Angstroms resolution structure of the P5CR apo enzyme, its 3.1, Angstroms resolution ternary complex with NAD(P)H and substrate-analog., The refined structures demonstrate a decameric architecture with five, homodimer subunits and ten catalytic sites arranged around a peripheral, circular groove. Mutagenesis and kinetic studies reveal the pivotal roles, of the dinucleotide-binding Rossmann motif and residue Glu221 in the human, enzyme. Human P5CR is thermostable and the crystals were grown at 37, degrees C. The enzyme is implicated in oxidation of the anti-tumor drug, thioproline.
+Pyrroline-5-carboxylate reductase (P5CR) is a universal housekeeping enzyme that catalyzes the reduction of Delta(1)-pyrroline-5-carboxylate (P5C) to proline using NAD(P)H as the cofactor. The enzymatic cycle between P5C and proline is very important for the regulation of amino acid metabolism, intracellular redox potential, and apoptosis. Here, we present the 2.8 Angstroms resolution structure of the P5CR apo enzyme, its 3.1 Angstroms resolution ternary complex with NAD(P)H and substrate-analog. The refined structures demonstrate a decameric architecture with five homodimer subunits and ten catalytic sites arranged around a peripheral circular groove. Mutagenesis and kinetic studies reveal the pivotal roles of the dinucleotide-binding Rossmann motif and residue Glu221 in the human enzyme. Human P5CR is thermostable and the crystals were grown at 37 degrees C. The enzyme is implicated in oxidation of the anti-tumor drug thioproline.
 ==About this Structure==
-GER is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Pyrroline-5-carboxylate_reductase Pyrroline-5-carboxylate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.2 1.5.1.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GER OCA].
+GER is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Pyrroline-5-carboxylate_reductase Pyrroline-5-carboxylate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.2 1.5.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GER OCA].
 ==Reference==
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 [[Category: oxidative mechanism of human pyrroline-5-carboxylate]]
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