2gdj: Difference between revisions

Revision as of 18:30, 21 February 2008

Delta-62 RADA recombinase in complex with AMP-PNP and magnesium

OverviewOverview

Proteins in the RecA/RadA/Rad51 family form helical filaments on DNA that function in homologous recombination. While these proteins all have the same highly conserved ATP binding core, the RadA/Rad51 proteins have an N-terminal domain that shows no homology with the C-terminal domain found in RecA. Both the Rad51 N-terminal and RecA C-terminal domains have been shown to bind DNA, but no role for these domains has been established. We show that RadA filaments can be trapped in either an inactive or active conformation with respect to the ATPase and that activation involves a large rotation of the subunit aided by the N-terminal domain. The G103E mutation within the yeast Rad51 N-terminal domain inactivates the filament by failing to make proper contacts between the N-terminal domain and the core. These results show that the N-terminal domains play a regulatory role in filament activation and highlight the modular architecture of the recombination proteins.

About this StructureAbout this Structure

2GDJ is a Single protein structure of sequence from Methanococcus voltae with and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

The Rad51/RadA N-terminal domain activates nucleoprotein filament ATPase activity., Galkin VE, Wu Y, Zhang XP, Qian X, He Y, Yu X, Heyer WD, Luo Y, Egelman EH, Structure. 2006 Jun;14(6):983-92. PMID:16765891

Page seeded by OCA on Thu Feb 21 17:30:41 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:2gdj.gif|left|200px]]<br /><applet load="2gdj" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2gdj.gif|left|200px]]<br /><applet load="2gdj" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2gdj, resolution 2.50&Aring;" />
 '''Delta-62 RADA recombinase in complex with AMP-PNP and magnesium'''<br />
 ==Overview==
-Proteins in the RecA/RadA/Rad51 family form helical filaments on DNA that, function in homologous recombination. While these proteins all have the, same highly conserved ATP binding core, the RadA/Rad51 proteins have an, N-terminal domain that shows no homology with the C-terminal domain found, in RecA. Both the Rad51 N-terminal and RecA C-terminal domains have been, shown to bind DNA, but no role for these domains has been established. We, show that RadA filaments can be trapped in either an inactive or active, conformation with respect to the ATPase and that activation involves a, large rotation of the subunit aided by the N-terminal domain. The G103E, mutation within the yeast Rad51 N-terminal domain inactivates the filament, by failing to make proper contacts between the N-terminal domain and the, core. These results show that the N-terminal domains play a regulatory, role in filament activation and highlight the modular architecture of the, recombination proteins.
+Proteins in the RecA/RadA/Rad51 family form helical filaments on DNA that function in homologous recombination. While these proteins all have the same highly conserved ATP binding core, the RadA/Rad51 proteins have an N-terminal domain that shows no homology with the C-terminal domain found in RecA. Both the Rad51 N-terminal and RecA C-terminal domains have been shown to bind DNA, but no role for these domains has been established. We show that RadA filaments can be trapped in either an inactive or active conformation with respect to the ATPase and that activation involves a large rotation of the subunit aided by the N-terminal domain. The G103E mutation within the yeast Rad51 N-terminal domain inactivates the filament by failing to make proper contacts between the N-terminal domain and the core. These results show that the N-terminal domains play a regulatory role in filament activation and highlight the modular architecture of the recombination proteins.
 ==About this Structure==
-GDJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanococcus_voltae Methanococcus voltae] with MG and ANP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GDJ OCA].
+GDJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanococcus_voltae Methanococcus voltae] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ANP:'>ANP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GDJ OCA].
 ==Reference==
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 [[Category: protein-atp complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:08:28 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:30:41 2008''