3pkm: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
{{STRUCTURE_3pkm| PDB=3pkm | SCENE= }} | {{STRUCTURE_3pkm| PDB=3pkm | SCENE= }} | ||
===Crystal structure of Cas6 with its substrate RNA=== | ===Crystal structure of Cas6 with its substrate RNA=== | ||
{{ABSTRACT_PUBMED_21300293}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/Q8U1S4_PYRFU Q8U1S4_PYRFU]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), also called psiRNA (prokaryotic silencing) in this organism. Processes pre-crRNA into individual crRNA units, with an 8-nt 5'-repeat DNA tag that may help other proteins recognize the crRNA. Further processing occurs at their 3' termini in this organism. Generates a 5' hydroxy and 2',3'-cyclic phosphodiester.<ref>PMID:20884784</ref> <ref>PMID:19141480</ref> | |||
==About this Structure== | ==About this Structure== | ||
| Line 22: | Line 10: | ||
==Reference== | ==Reference== | ||
<ref group="xtra">PMID: | <ref group="xtra">PMID:021300293</ref><references group="xtra"/><references/> | ||
[[Category: Pyrococcus furiosus]] | [[Category: Pyrococcus furiosus]] | ||
[[Category: Li, H.]] | [[Category: Li, H.]] | ||
[[Category: Preamplume, G.]] | [[Category: Preamplume, G.]] | ||
[[Category: Wang, R.]] | [[Category: Wang, R.]] | ||
[[Category: Cas6]] | |||
[[Category: Crispr]] | |||
[[Category: Crispr processing endonuclease]] | |||
[[Category: Endonuclease]] | |||
[[Category: Ferridoxin fold]] | |||
[[Category: Hydrolase-rna complex]] | |||
[[Category: Repeat rna]] | |||
Revision as of 11:08, 8 May 2013
Crystal structure of Cas6 with its substrate RNACrystal structure of Cas6 with its substrate RNA
Template:ABSTRACT PUBMED 21300293
FunctionFunction
[Q8U1S4_PYRFU] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), also called psiRNA (prokaryotic silencing) in this organism. Processes pre-crRNA into individual crRNA units, with an 8-nt 5'-repeat DNA tag that may help other proteins recognize the crRNA. Further processing occurs at their 3' termini in this organism. Generates a 5' hydroxy and 2',3'-cyclic phosphodiester.[1] [2]
About this StructureAbout this Structure
3pkm is a 4 chain structure with sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.
ReferenceReference
- ↑ Wang R, Preamplume G, Terns MP, Terns RM, Li H. Interaction of the Cas6 Riboendonuclease with CRISPR RNAs: Recognition and Cleavage. Structure. 2011 Feb 9;19(2):257-64. PMID:21300293 doi:10.1016/j.str.2010.11.014
- ↑ Carte J, Pfister NT, Compton MM, Terns RM, Terns MP. Binding and cleavage of CRISPR RNA by Cas6. RNA. 2010 Nov;16(11):2181-8. doi: 10.1261/rna.2230110. Epub 2010 Sep 30. PMID:20884784 doi:10.1261/rna.2230110
- ↑ Carte J, Wang R, Li H, Terns RM, Terns MP. Cas6 is an endoribonuclease that generates guide RNAs for invader defense in prokaryotes. Genes Dev. 2008 Dec 15;22(24):3489-96. PMID:19141480 doi:22/24/3489