2g9t: Difference between revisions

Revision as of 18:29, 21 February 2008

Crystal structure of the SARS coronavirus nsp10 at 2.1A

OverviewOverview

The severe acute respiratory syndrome coronavirus (SARS-CoV) nonstructural proteins nsp1 to nsp16 have been implicated by genetic analysis in the assembly of a functional replication/transcription complex. We report the crystal structure of nsp10 from SARS-CoV at 2.1-A resolution. The nsp10 structure has a novel fold, and 12 identical subunits assemble to form a unique spherical dodecameric architecture. Two zinc fingers have been identified from the nsp10 monomer structure with the sequence motifs C-(X)2-C-(X)5-H-(X)6-C and C-(X)2-C-(X)7-C-(X)-C. The nsp10 crystal structure is the first of a new class of zinc finger protein three-dimensional structures to be revealed experimentally. The zinc finger sequence motifs are conserved among all three coronavirus antigenic groups, implicating an essential function for nsp10 in all coronaviruses. Based on the structure, we propose that nsp10 is a transcription factor for coronavirus replication/transcription.

About this StructureAbout this Structure

2G9T is a Single protein structure of sequence from Human sars coronavirus with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Dodecamer structure of severe acute respiratory syndrome coronavirus nonstructural protein nsp10., Su D, Lou Z, Sun F, Zhai Y, Yang H, Zhang R, Joachimiak A, Zhang XC, Bartlam M, Rao Z, J Virol. 2006 Aug;80(16):7902-8. PMID:16873247

Page seeded by OCA on Thu Feb 21 17:29:33 2008

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OCA

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-[[Image:2g9t.gif|left|200px]]<br /><applet load="2g9t" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2g9t.gif|left|200px]]<br /><applet load="2g9t" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2g9t, resolution 2.1&Aring;" />
 '''Crystal structure of the SARS coronavirus nsp10 at 2.1A'''<br />
 ==Overview==
-The severe acute respiratory syndrome coronavirus (SARS-CoV) nonstructural, proteins nsp1 to nsp16 have been implicated by genetic analysis in the, assembly of a functional replication/transcription complex. We report the, crystal structure of nsp10 from SARS-CoV at 2.1-A resolution. The nsp10, structure has a novel fold, and 12 identical subunits assemble to form a, unique spherical dodecameric architecture. Two zinc fingers have been, identified from the nsp10 monomer structure with the sequence motifs, C-(X)2-C-(X)5-H-(X)6-C and C-(X)2-C-(X)7-C-(X)-C. The nsp10 crystal, structure is the first of a new class of zinc finger protein, three-dimensional structures to be revealed experimentally. The zinc, finger sequence motifs are conserved among all three coronavirus antigenic, groups, implicating an essential function for nsp10 in all coronaviruses., Based on the structure, we propose that nsp10 is a transcription factor, for coronavirus replication/transcription.
+The severe acute respiratory syndrome coronavirus (SARS-CoV) nonstructural proteins nsp1 to nsp16 have been implicated by genetic analysis in the assembly of a functional replication/transcription complex. We report the crystal structure of nsp10 from SARS-CoV at 2.1-A resolution. The nsp10 structure has a novel fold, and 12 identical subunits assemble to form a unique spherical dodecameric architecture. Two zinc fingers have been identified from the nsp10 monomer structure with the sequence motifs C-(X)2-C-(X)5-H-(X)6-C and C-(X)2-C-(X)7-C-(X)-C. The nsp10 crystal structure is the first of a new class of zinc finger protein three-dimensional structures to be revealed experimentally. The zinc finger sequence motifs are conserved among all three coronavirus antigenic groups, implicating an essential function for nsp10 in all coronaviruses. Based on the structure, we propose that nsp10 is a transcription factor for coronavirus replication/transcription.
 ==About this Structure==
-G9T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2G9T OCA].
+G9T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G9T OCA].
 ==Reference==
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 [[Category: sars]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:03:55 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:29:33 2008''