2g6y: Difference between revisions

Revision as of 18:28, 21 February 2008

Crystal structure of the novel green fluorescent protein from marine copepod Pontellina plumata

OverviewOverview

Since the cloning of Aequorea victoria green fluorescent protein (GFP) in 1992, a family of known GFP-like proteins has been growing rapidly. Today, it includes more than a hundred proteins with different spectral characteristics cloned from Cnidaria species. For some of these proteins, crystal structures have been solved, showing diversity in chromophore modifications and conformational states. However, we are still far from a complete understanding of the origin, functions and evolution of the GFP family. Novel proteins of the family were recently cloned from evolutionarily distant marine Copepoda species, phylum Arthropoda, demonstrating an extremely rapid generation of fluorescent signal. Here, we have generated a non-aggregating mutant of Copepoda fluorescent protein and solved its high-resolution crystal structure. It was found that the protein beta-barrel contains a pore, leading to the chromophore. Using site-directed mutagenesis, we showed that this feature is critical for the fast maturation of the chromophore.

About this StructureAbout this Structure

2G6Y is a Single protein structure of sequence from Pontellina plumata. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the fast maturation of Arthropoda green fluorescent protein., Evdokimov AG, Pokross ME, Egorov NS, Zaraisky AG, Yampolsky IV, Merzlyak EM, Shkoporov AN, Sander I, Lukyanov KA, Chudakov DM, EMBO Rep. 2006 Oct;7(10):1006-12. Epub 2006 Aug 25. PMID:16936637

Page seeded by OCA on Thu Feb 21 17:28:44 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 4: / Line 4: @@
 ==Overview==
-Since the cloning of Aequorea victoria green fluorescent protein (GFP) in, 1992, a family of known GFP-like proteins has been growing rapidly. Today, it includes more than a hundred proteins with different spectral, characteristics cloned from Cnidaria species. For some of these proteins, crystal structures have been solved, showing diversity in chromophore, modifications and conformational states. However, we are still far from a, complete understanding of the origin, functions and evolution of the GFP, family. Novel proteins of the family were recently cloned from, evolutionarily distant marine Copepoda species, phylum Arthropoda, demonstrating an extremely rapid generation of fluorescent signal. Here, we have generated a non-aggregating mutant of Copepoda fluorescent protein, and solved its high-resolution crystal structure. It was found that the, protein beta-barrel contains a pore, leading to the chromophore. Using, site-directed mutagenesis, we showed that this feature is critical for the, fast maturation of the chromophore.
+Since the cloning of Aequorea victoria green fluorescent protein (GFP) in 1992, a family of known GFP-like proteins has been growing rapidly. Today, it includes more than a hundred proteins with different spectral characteristics cloned from Cnidaria species. For some of these proteins, crystal structures have been solved, showing diversity in chromophore modifications and conformational states. However, we are still far from a complete understanding of the origin, functions and evolution of the GFP family. Novel proteins of the family were recently cloned from evolutionarily distant marine Copepoda species, phylum Arthropoda, demonstrating an extremely rapid generation of fluorescent signal. Here, we have generated a non-aggregating mutant of Copepoda fluorescent protein and solved its high-resolution crystal structure. It was found that the protein beta-barrel contains a pore, leading to the chromophore. Using site-directed mutagenesis, we showed that this feature is critical for the fast maturation of the chromophore.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Pontellina plumata]]
 [[Category: Single protein]]
-[[Category: Chudakov, D.M.]]
+[[Category: Chudakov, D M.]]
-[[Category: Evdokimov, A.G.]]
+[[Category: Evdokimov, A G.]]
-[[Category: Pokross, M.E.]]
+[[Category: Pokross, M E.]]
 [[Category: beta-can]]
 [[Category: green fluorescent protein]]
@@ Line 21: / Line 21: @@
 [[Category: rapid maturation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:53:11 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:28:44 2008''