G1SecL05: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Moazzam Khan, Supriya Kannan, Rubab Farhat, Noopoor Akruwala, Michal Harel

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 <scene name='Studio:G1SecL01/2/2'>Three Loops</scene>
-LA-2 fab recognizes the three surface-exposed loops; loop1, loop 2 and loop 3 of the C-terminal domain of OspA that are on the tip of the elongated molecule most distant from the lipid-modified N terminus. Residues 203 to 220 in “loop 1“,residues 224 to 233 in “loop 2“ and residues 246 to 257 in “loop 3“are mostly effected by la-2 binding. In loop 1 residues 206 and 216 are not affected. The interactions between OspA and LA-2 include eight direct hydrogen bonds, four solvent-bridged hydrogen bonds, three ion pairs, and numerous van der Waals interactions.<ref name = "interactions">•Ding W, Huang X, Yang X, Dunn JJ, Luft BJ, Koide S, Lawson CL. Structural identification of a key protective B-cell epitope in Lyme disease antigen OspA. J Mol Biol. 2000 Oct 6;302(5):1153-64.PMID:11183781 doi:10.1006/jmbi.2000.4119
+LA-2 fab recognizes the three surface-exposed loops; loop1, loop 2 and loop 3 of the C-terminal domain of OspA that are on the tip of the elongated molecule most distant from the lipid-modified N terminus. Residues 203 to 220 in “loop 1“,residues 224 to 233 in “loop 2“ and residues 246 to 257 in “loop 3“are mostly effected by la-2 binding. In loop 1 residues 206 and 216 are not affected. The interactions between OspA and LA-2 include eight direct hydrogen bonds, four solvent-bridged hydrogen bonds, three ion pairs, and numerous van der Waals interactions.LA-2 recognition of OspA involves an induced fit mechanism where loop 1-3 conformations shift to optimize complementarity to the antigen-combining site. <ref name = "interactions">•Ding W, Huang X, Yang X, Dunn JJ, Luft BJ, Koide S, Lawson CL. Structural identification of a key protective B-cell epitope in Lyme disease antigen OspA. J Mol Biol. 2000 Oct 6;302(5):1153-64.PMID:11183781 doi:10.1006/jmbi.2000.4119
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