2fxl: Difference between revisions

Revision as of 18:26, 21 February 2008

Urate oxidase from aspergillus flavus complexed with allantoin

OverviewOverview

Urate oxidase from Aspergillus flavus catalyzes the degradation of uric acid to [S]-allantoin through 5-hydroxyisourate as a metastable intermediate. The second degradation step is thought either catalyzed by another specific enzyme, or spontaneous. The structure of the enzyme was known at high resolution by X-ray diffraction of I222 crystals complexed with a purine-type inhibitor (8-azaxanthin). Analyzing the X-ray structure of urate oxidase treated with an excess of urate, the natural substrate, shows unexpectedly that the active site recaptures [S]-allantoin from the racemic end product of a second degradation step.

About this StructureAbout this Structure

2FXL is a Single protein structure of sequence from Aspergillus flavus with as ligand. Active as Urate oxidase, with EC number 1.7.3.3 Full crystallographic information is available from OCA.

ReferenceReference

Recapture of [S]-allantoin, the product of the two-step degradation of uric acid, by urate oxidase., Gabison L, Chiadmi M, Colloc'h N, Castro B, El Hajji M, Prange T, FEBS Lett. 2006 Apr 3;580(8):2087-91. Epub 2006 Mar 10. PMID:16545381

Page seeded by OCA on Thu Feb 21 17:26:06 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:2fxl.gif|left|200px]]<br /><applet load="2fxl" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2fxl.gif|left|200px]]<br /><applet load="2fxl" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2fxl, resolution 1.76&Aring;" />
 '''Urate oxidase from aspergillus flavus complexed with allantoin'''<br />
 ==Overview==
-Urate oxidase from Aspergillus flavus catalyzes the degradation of uric, acid to [S]-allantoin through 5-hydroxyisourate as a metastable, intermediate. The second degradation step is thought either catalyzed by, another specific enzyme, or spontaneous. The structure of the enzyme was, known at high resolution by X-ray diffraction of I222 crystals complexed, with a purine-type inhibitor (8-azaxanthin). Analyzing the X-ray structure, of urate oxidase treated with an excess of urate, the natural substrate, shows unexpectedly that the active site recaptures [S]-allantoin from the, racemic end product of a second degradation step.
+Urate oxidase from Aspergillus flavus catalyzes the degradation of uric acid to [S]-allantoin through 5-hydroxyisourate as a metastable intermediate. The second degradation step is thought either catalyzed by another specific enzyme, or spontaneous. The structure of the enzyme was known at high resolution by X-ray diffraction of I222 crystals complexed with a purine-type inhibitor (8-azaxanthin). Analyzing the X-ray structure of urate oxidase treated with an excess of urate, the natural substrate, shows unexpectedly that the active site recaptures [S]-allantoin from the racemic end product of a second degradation step.
 ==About this Structure==
-FXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus] with 2AL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Urate_oxidase Urate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FXL OCA].
+FXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus] with <scene name='pdbligand=2AL:'>2AL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Urate_oxidase Urate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FXL OCA].
 ==Reference==
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 [[Category: Chiadmi, M.]]
 [[Category: Gabison, L.]]
-[[Category: H, N.Colloc.]]
+[[Category: H, N Colloc.]]
 [[Category: Prange, T.]]
 [[Category: 2AL]]
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 [[Category: uric acid degradation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:49:31 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:26:06 2008''