2fub: Difference between revisions
New page: left|200px<br /><applet load="2fub" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fub, resolution 2.30Å" /> '''Crystal structure of... |
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[[Image:2fub.gif|left|200px]]<br /><applet load="2fub" size=" | [[Image:2fub.gif|left|200px]]<br /><applet load="2fub" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2fub, resolution 2.30Å" /> | caption="2fub, resolution 2.30Å" /> | ||
'''Crystal structure of urate oxidase at 140 MPa'''<br /> | '''Crystal structure of urate oxidase at 140 MPa'''<br /> | ||
==Overview== | ==Overview== | ||
We report the three-dimensional structure determined by high-pressure | We report the three-dimensional structure determined by high-pressure macromolecular crystallography (HPMX) of a 135-kDa homo-tetrameric enzyme, urate oxidase from Aspergillus flavus complexed with its potent inhibitor 8-azaxanthin. Urate oxidase crystals are quite sensitive to pressure, as three-dimensional order is lost at about 180 MPa. A highly complete 2.3 A resolution data set was collected at 140 MPa, close to the critical pressure. Crystal structures at atmospheric pressure and at high pressure were refined in the orthorhombic space group I222 with final crystallographic R factors 14.1% and 16.1%, respectively. The effect of pressure on temperature factors, ordered water molecules, hydrogen bond lengths, contacts, buried surface areas as well as cavity volume was investigated. Results suggest that the onset of disruption of the tetrameric assembly by pressure has been captured in the crystalline state. | ||
==About this Structure== | ==About this Structure== | ||
2FUB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus] with AZA and CYS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Urate_oxidase Urate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] Full crystallographic information is available from [http:// | 2FUB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus] with <scene name='pdbligand=AZA:'>AZA</scene> and <scene name='pdbligand=CYS:'>CYS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Urate_oxidase Urate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FUB OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Fourme, R.]] | [[Category: Fourme, R.]] | ||
[[Category: Girard, E.]] | [[Category: Girard, E.]] | ||
[[Category: h, N | [[Category: h, N Colloc.]] | ||
[[Category: AZA]] | [[Category: AZA]] | ||
[[Category: CYS]] | [[Category: CYS]] | ||
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[[Category: tetramer]] | [[Category: tetramer]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:12 2008'' | ||
Revision as of 18:25, 21 February 2008
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Crystal structure of urate oxidase at 140 MPa
OverviewOverview
We report the three-dimensional structure determined by high-pressure macromolecular crystallography (HPMX) of a 135-kDa homo-tetrameric enzyme, urate oxidase from Aspergillus flavus complexed with its potent inhibitor 8-azaxanthin. Urate oxidase crystals are quite sensitive to pressure, as three-dimensional order is lost at about 180 MPa. A highly complete 2.3 A resolution data set was collected at 140 MPa, close to the critical pressure. Crystal structures at atmospheric pressure and at high pressure were refined in the orthorhombic space group I222 with final crystallographic R factors 14.1% and 16.1%, respectively. The effect of pressure on temperature factors, ordered water molecules, hydrogen bond lengths, contacts, buried surface areas as well as cavity volume was investigated. Results suggest that the onset of disruption of the tetrameric assembly by pressure has been captured in the crystalline state.
About this StructureAbout this Structure
2FUB is a Single protein structure of sequence from Aspergillus flavus with and as ligands. Active as Urate oxidase, with EC number 1.7.3.3 Full crystallographic information is available from OCA.
ReferenceReference
High pressure macromolecular crystallography: the 140-MPa crystal structure at 2.3 A resolution of urate oxidase, a 135-kDa tetrameric assembly., Colloc'h N, Girard E, Dhaussy AC, Kahn R, Ascone I, Mezouar M, Fourme R, Biochim Biophys Acta. 2006 Mar;1764(3):391-7. Epub 2006 Jan 26. PMID:16478683
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