2fd2: Difference between revisions

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CRYSTALLOGRAPHIC ANALYSIS OF TWO SITE-DIRECTED MUTANTS OF AZOTOBACTER VINELANDII FERREDOXIN

OverviewOverview

The crystal structure of the C24A mutant of Azotobacter vinelandii 7Fe ferredoxin (FdI) has been solved and refined at 2.0-A resolution. The structure is isomorphous to native FdI except at the site of mutation where A24 moves toward the [4Fe-4S] cluster. In spite of this inefficient packing results: three of five van der Waals contacts from the S gamma of C24 in native FdI are lost and the remaining two become longer. Consequently, the [4Fe-4S] cluster is either disordered or has a higher temperature factor (B factor) compared to the rest of the C24A FdI molecule. In addition, the entire C24A FdI structure has a higher overall B factor than native FdI. Therefore, in comparison to native FdI, the C24A mutant is isomorphous but exhibits large differences in B factor, especially at the [4Fe-4S] cluster. In contrast, the C20A FdI structure (Martin, A. G., Burgess, B. K., Stout, C. D., Cash, V. L., Dean, D. R., Jensen, G. M., and Stephens, P. J. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 598-602), which contains large structural rearrangements in the vicinity of the [4Fe-4S] cluster, exhibits essentially no change in B factor. The conformational change observed at residue 24 is similar in both C24A and C20A FdI structures. The solvent accessibility of the Fe atoms in the [3Fe-4S] and [4Fe-4S] clusters is similar in C24A, C20A, and native FdI.

About this StructureAbout this Structure

2FD2 is a Single protein structure of sequence from Azotobacter vinelandii with and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Crystallographic analysis of two site-directed mutants of Azotobacter vinelandii ferredoxin., Soman J, Iismaa S, Stout CD, J Biol Chem. 1991 Nov 15;266(32):21558-62. PMID:1939185

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-[[Image:2fd2.jpg|left|200px]]<br /><applet load="2fd2" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2fd2.jpg|left|200px]]<br /><applet load="2fd2" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2fd2, resolution 1.9&Aring;" />
 '''CRYSTALLOGRAPHIC ANALYSIS OF TWO SITE-DIRECTED MUTANTS OF AZOTOBACTER VINELANDII FERREDOXIN'''<br />
 ==Overview==
-The crystal structure of the C24A mutant of Azotobacter vinelandii 7Fe, ferredoxin (FdI) has been solved and refined at 2.0-A resolution. The, structure is isomorphous to native FdI except at the site of mutation, where A24 moves toward the [4Fe-4S] cluster. In spite of this inefficient, packing results: three of five van der Waals contacts from the S gamma of, C24 in native FdI are lost and the remaining two become longer., Consequently, the [4Fe-4S] cluster is either disordered or has a higher, temperature factor (B factor) compared to the rest of the C24A FdI, molecule. In addition, the entire C24A FdI structure has a higher overall, B factor than native FdI. Therefore, in comparison to native FdI, the C24A, mutant is isomorphous but exhibits large differences in B factor, especially at the [4Fe-4S] cluster. In contrast, the C20A FdI structure, (Martin, A. G., Burgess, B. K., Stout, C. D., Cash, V. L., Dean, D. R., Jensen, G. M., and Stephens, P. J. (1990) Proc. Natl. Acad. Sci. U. S. A., 87, 598-602), which contains large structural rearrangements in the, vicinity of the [4Fe-4S] cluster, exhibits essentially no change in B, factor. The conformational change observed at residue 24 is similar in, both C24A and C20A FdI structures. The solvent accessibility of the Fe, atoms in the [3Fe-4S] and [4Fe-4S] clusters is similar in C24A, C20A, and, native FdI.
+The crystal structure of the C24A mutant of Azotobacter vinelandii 7Fe ferredoxin (FdI) has been solved and refined at 2.0-A resolution. The structure is isomorphous to native FdI except at the site of mutation where A24 moves toward the [4Fe-4S] cluster. In spite of this inefficient packing results: three of five van der Waals contacts from the S gamma of C24 in native FdI are lost and the remaining two become longer. Consequently, the [4Fe-4S] cluster is either disordered or has a higher temperature factor (B factor) compared to the rest of the C24A FdI molecule. In addition, the entire C24A FdI structure has a higher overall B factor than native FdI. Therefore, in comparison to native FdI, the C24A mutant is isomorphous but exhibits large differences in B factor, especially at the [4Fe-4S] cluster. In contrast, the C20A FdI structure (Martin, A. G., Burgess, B. K., Stout, C. D., Cash, V. L., Dean, D. R., Jensen, G. M., and Stephens, P. J. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 598-602), which contains large structural rearrangements in the vicinity of the [4Fe-4S] cluster, exhibits essentially no change in B factor. The conformational change observed at residue 24 is similar in both C24A and C20A FdI structures. The solvent accessibility of the Fe atoms in the [3Fe-4S] and [4Fe-4S] clusters is similar in C24A, C20A, and native FdI.
 ==About this Structure==
-FD2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with SF4 and F3S as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FD2 OCA].
+FD2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with <scene name='pdbligand=SF4:'>SF4</scene> and <scene name='pdbligand=F3S:'>F3S</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FD2 OCA].
 ==Reference==
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 [[Category: Azotobacter vinelandii]]
 [[Category: Single protein]]
-[[Category: Stout, C.D.]]
+[[Category: Stout, C D.]]
 [[Category: F3S]]
 [[Category: SF4]]
 [[Category: electron transfer(iron-sulfur protein)]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:29:13 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:20:06 2008''