2f52: Difference between revisions
New page: left|200px<br /><applet load="2f52" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f52" /> '''Solution structure of cold shock protein Csp... |
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'''Solution structure of cold shock protein CspB from Bacillus subtilis in complex with heptathymidine'''<br /> | '''Solution structure of cold shock protein CspB from Bacillus subtilis in complex with heptathymidine'''<br /> | ||
==Overview== | ==Overview== | ||
Cold shock proteins (CSP) belong to the family of single-stranded nucleic | Cold shock proteins (CSP) belong to the family of single-stranded nucleic acid binding proteins with OB-fold. CSP are believed to function as 'RNA chaperones' and during anti-termination. We determined the solution structure of Bs-CspB bound to the single-stranded DNA (ssDNA) fragment heptathymidine (dT7) by NMR spectroscopy. Bs-CspB reveals an almost invariant conformation when bound to dT7 with only minor reorientations in loop beta1-beta2 and beta3-beta4 and of few aromatic side chains involved in base stacking. Binding studies of protein variants and mutated ssDNA demonstrated that Bs-CspB associates with ssDNA at almost diffusion controlled rates and low sequence specificity consistent with its biological function. A variation of the ssDNA affinity is accomplished solely by changes of the dissociation rate. 15N NMR relaxation and H/D exchange experiments revealed that binding of dT7 increases the stability of Bs-CspB and reduces the sub-nanosecond dynamics of the entire protein and especially of loop beta3-beta4. | ||
==About this Structure== | ==About this Structure== | ||
2F52 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http:// | 2F52 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F52 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: ob-fold]] | [[Category: ob-fold]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:17:45 2008'' | ||
Revision as of 18:17, 21 February 2008
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Solution structure of cold shock protein CspB from Bacillus subtilis in complex with heptathymidine
OverviewOverview
Cold shock proteins (CSP) belong to the family of single-stranded nucleic acid binding proteins with OB-fold. CSP are believed to function as 'RNA chaperones' and during anti-termination. We determined the solution structure of Bs-CspB bound to the single-stranded DNA (ssDNA) fragment heptathymidine (dT7) by NMR spectroscopy. Bs-CspB reveals an almost invariant conformation when bound to dT7 with only minor reorientations in loop beta1-beta2 and beta3-beta4 and of few aromatic side chains involved in base stacking. Binding studies of protein variants and mutated ssDNA demonstrated that Bs-CspB associates with ssDNA at almost diffusion controlled rates and low sequence specificity consistent with its biological function. A variation of the ssDNA affinity is accomplished solely by changes of the dissociation rate. 15N NMR relaxation and H/D exchange experiments revealed that binding of dT7 increases the stability of Bs-CspB and reduces the sub-nanosecond dynamics of the entire protein and especially of loop beta3-beta4.
About this StructureAbout this Structure
2F52 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
ReferenceReference
Recognition of T-rich single-stranded DNA by the cold shock protein Bs-CspB in solution., Zeeb M, Max KE, Weininger U, Low C, Sticht H, Balbach J, Nucleic Acids Res. 2006;34(16):4561-71. Epub 2006 Sep 6. PMID:16956971
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