2f03: Difference between revisions

Revision as of 18:16, 21 February 2008

Crystal structure of tetrameric restriction endonuclease SfiI in complex with cognate DNA (partial bound form)

OverviewOverview

Many reactions in cells proceed via the sequestration of two DNA molecules in a synaptic complex. SfiI is a member of a growing family of restriction enzymes that can bind and cleave two DNA sites simultaneously. We present here the structures of tetrameric SfiI in complex with cognate DNA. The structures reveal two different binding states of SfiI: one with both DNA-binding sites fully occupied and the other with fully and partially occupied sites. These two states provide details on how SfiI recognizes and cleaves its target DNA sites, and gives insight into sequential binding events. The SfiI recognition sequence (GGCCNNNN[downward arrow]NGGCC) is a subset of the recognition sequence of BglI (GCCNNNN[downward arrow]NGGC), and both enzymes cleave their target DNAs to leave 3-base 3' overhangs. We show that even though SfiI is a tetramer and BglI is a dimer, and there is little sequence similarity between the two enzymes, their modes of DNA recognition are unusually similar.

About this StructureAbout this Structure

2F03 is a Single protein structure of sequence from [1] with as ligand. Active as Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 Full crystallographic information is available from OCA.

ReferenceReference

A view of consecutive binding events from structures of tetrameric endonuclease SfiI bound to DNA., Vanamee ES, Viadiu H, Kucera R, Dorner L, Picone S, Schildkraut I, Aggarwal AK, EMBO J. 2005 Dec 7;24(23):4198-208. Epub 2005 Nov 24. PMID:16308566

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-[[Image:2f03.jpg|left|200px]]<br /><applet load="2f03" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2f03.jpg|left|200px]]<br /><applet load="2f03" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2f03, resolution 3.050&Aring;" />
 '''Crystal structure of tetrameric restriction endonuclease SfiI in complex with cognate DNA (partial bound form)'''<br />
 ==Overview==
-Many reactions in cells proceed via the sequestration of two DNA molecules, in a synaptic complex. SfiI is a member of a growing family of restriction, enzymes that can bind and cleave two DNA sites simultaneously. We present, here the structures of tetrameric SfiI in complex with cognate DNA. The, structures reveal two different binding states of SfiI: one with both, DNA-binding sites fully occupied and the other with fully and partially, occupied sites. These two states provide details on how SfiI recognizes, and cleaves its target DNA sites, and gives insight into sequential, binding events. The SfiI recognition sequence (GGCCNNNN[downward, arrow]NGGCC) is a subset of the recognition sequence of BglI, (GCCNNNN[downward arrow]NGGC), and both enzymes cleave their target DNAs, to leave 3-base 3' overhangs. We show that even though SfiI is a tetramer, and BglI is a dimer, and there is little sequence similarity between the, two enzymes, their modes of DNA recognition are unusually similar.
+Many reactions in cells proceed via the sequestration of two DNA molecules in a synaptic complex. SfiI is a member of a growing family of restriction enzymes that can bind and cleave two DNA sites simultaneously. We present here the structures of tetrameric SfiI in complex with cognate DNA. The structures reveal two different binding states of SfiI: one with both DNA-binding sites fully occupied and the other with fully and partially occupied sites. These two states provide details on how SfiI recognizes and cleaves its target DNA sites, and gives insight into sequential binding events. The SfiI recognition sequence (GGCCNNNN[downward arrow]NGGCC) is a subset of the recognition sequence of BglI (GCCNNNN[downward arrow]NGGC), and both enzymes cleave their target DNAs to leave 3-base 3' overhangs. We show that even though SfiI is a tetramer and BglI is a dimer, and there is little sequence similarity between the two enzymes, their modes of DNA recognition are unusually similar.
 ==About this Structure==
-F03 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2F03 OCA].
+F03 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F03 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Type II site-specific deoxyribonuclease]]
-[[Category: Aggarwal, A.K.]]
+[[Category: Aggarwal, A K.]]
-[[Category: Vanamee, E.S.]]
+[[Category: Vanamee, E S.]]
 [[Category: Viadiu, H]]
 [[Category: CA]]
@@ Line 21: / Line 21: @@
 [[Category: type iif restriction endonuclease]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:15:49 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:16:16 2008''