2eh8: Difference between revisions
New page: left|200px<br /><applet load="2eh8" size="350" color="white" frame="true" align="right" spinBox="true" caption="2eh8, resolution 2.60Å" /> '''Crystal structure of... |
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==Overview== | ==Overview== | ||
The humanized monoclonal antibody HzKR127 recognizes the preS1 domain of | The humanized monoclonal antibody HzKR127 recognizes the preS1 domain of the human hepatitis B virus surface proteins with a broadly neutralizing activity in vivo. We present the crystal structures of HzKR127 Fab and its complex with a major epitope peptide. In the complex structure, the bound peptide forms a type IV beta-turn followed by 3(10) helical turn, the looped-out conformation of which provides a structural basis for broad neutralization. Upon peptide binding, the antibody undergoes a dramatic complementarity determining region H3 lid opening. To understand the structural implication of the virus neutralization, we carried out comprehensive alanine-scanning mutagenesis of all complementarity determining region residues in HzKR127 Fab. The functional mapping of the antigen-combining site demonstrates the specific roles of major binding determinants in antigen binding, contributing to the rational design for maximal humanization and affinity maturation of the antibody. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Chi, S | [[Category: Chi, S W.]] | ||
[[Category: Hong, H | [[Category: Hong, H J.]] | ||
[[Category: Kim, S | [[Category: Kim, S J.]] | ||
[[Category: Maeng, C | [[Category: Maeng, C Y.]] | ||
[[Category: Ryu, S | [[Category: Ryu, S E.]] | ||
[[Category: crystal structure]] | [[Category: crystal structure]] | ||
[[Category: hepatitis b virus]] | [[Category: hepatitis b virus]] | ||
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[[Category: pres1]] | [[Category: pres1]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:10:03 2008'' | ||
Revision as of 18:10, 21 February 2008
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Crystal structure of the complex of humanized KR127 fab and PRES1 peptide epitope
OverviewOverview
The humanized monoclonal antibody HzKR127 recognizes the preS1 domain of the human hepatitis B virus surface proteins with a broadly neutralizing activity in vivo. We present the crystal structures of HzKR127 Fab and its complex with a major epitope peptide. In the complex structure, the bound peptide forms a type IV beta-turn followed by 3(10) helical turn, the looped-out conformation of which provides a structural basis for broad neutralization. Upon peptide binding, the antibody undergoes a dramatic complementarity determining region H3 lid opening. To understand the structural implication of the virus neutralization, we carried out comprehensive alanine-scanning mutagenesis of all complementarity determining region residues in HzKR127 Fab. The functional mapping of the antigen-combining site demonstrates the specific roles of major binding determinants in antigen binding, contributing to the rational design for maximal humanization and affinity maturation of the antibody.
About this StructureAbout this Structure
2EH8 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Broadly neutralizing anti-hepatitis B virus antibody reveals a complementarity determining region H3 lid-opening mechanism., Chi SW, Maeng CY, Kim SJ, Oh MS, Ryu CJ, Kim SJ, Han KH, Hong HJ, Ryu SE, Proc Natl Acad Sci U S A. 2007 May 29;104(22):9230-5. Epub 2007 May 17. PMID:17517649
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