2e4u: Difference between revisions

Revision as of 18:06, 21 February 2008

Crystal structure of the extracellular region of the group II metabotropic glutamate receptor complexed with L-glutamate

OverviewOverview

Metabotropic glutamate receptors play major roles in the activation of excitatory synapses in the central nerve system. We determined the crystal structure of the entire extracellular region of the group II receptor and that of the ligand-binding region of the group III receptor. A comparison among groups I, II, and III provides the structural basis that could account for the discrimination of group-specific agonists. Furthermore, the structure of group II includes the cysteine-rich domain, which is tightly linked to the ligand-binding domain by a disulfide bridge, suggesting a potential role in transmitting a ligand-induced conformational change into the downstream transmembrane region. The structure also reveals the lateral interaction between the two cysteine-rich domains, which could stimulate clustering of the dimeric receptors on the cell surface. We propose a general activation mechanism of the dimeric receptor coupled with both ligand-binding and interprotomer rearrangements.

About this StructureAbout this Structure

2E4U is a Single protein structure of sequence from Rattus norvegicus with and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Structures of the extracellular regions of the group II/III metabotropic glutamate receptors., Muto T, Tsuchiya D, Morikawa K, Jingami H, Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):3759-64. Epub 2007 Feb 26. PMID:17360426

Page seeded by OCA on Thu Feb 21 17:05:59 2008

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OCA

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-[[Image:2e4u.gif|left|200px]]<br /><applet load="2e4u" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2e4u.gif|left|200px]]<br /><applet load="2e4u" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2e4u, resolution 2.35&Aring;" />
 '''Crystal structure of the extracellular region of the group II metabotropic glutamate receptor complexed with L-glutamate'''<br />
 ==Overview==
-Metabotropic glutamate receptors play major roles in the activation of, excitatory synapses in the central nerve system. We determined the crystal, structure of the entire extracellular region of the group II receptor and, that of the ligand-binding region of the group III receptor. A comparison, among groups I, II, and III provides the structural basis that could, account for the discrimination of group-specific agonists. Furthermore, the structure of group II includes the cysteine-rich domain, which is, tightly linked to the ligand-binding domain by a disulfide bridge, suggesting a potential role in transmitting a ligand-induced, conformational change into the downstream transmembrane region. The, structure also reveals the lateral interaction between the two, cysteine-rich domains, which could stimulate clustering of the dimeric, receptors on the cell surface. We propose a general activation mechanism, of the dimeric receptor coupled with both ligand-binding and interprotomer, rearrangements.
+Metabotropic glutamate receptors play major roles in the activation of excitatory synapses in the central nerve system. We determined the crystal structure of the entire extracellular region of the group II receptor and that of the ligand-binding region of the group III receptor. A comparison among groups I, II, and III provides the structural basis that could account for the discrimination of group-specific agonists. Furthermore, the structure of group II includes the cysteine-rich domain, which is tightly linked to the ligand-binding domain by a disulfide bridge, suggesting a potential role in transmitting a ligand-induced conformational change into the downstream transmembrane region. The structure also reveals the lateral interaction between the two cysteine-rich domains, which could stimulate clustering of the dimeric receptors on the cell surface. We propose a general activation mechanism of the dimeric receptor coupled with both ligand-binding and interprotomer rearrangements.
 ==About this Structure==
-E4U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with NAG and GLU as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2E4U OCA].
+E4U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=NAG:'>NAG</scene> and <scene name='pdbligand=GLU:'>GLU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E4U OCA].
 ==Reference==
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 [[Category: neuron]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:56:47 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:05:59 2008''