2duu: Difference between revisions
New page: left|200px<br /><applet load="2duu" size="350" color="white" frame="true" align="right" spinBox="true" caption="2duu, resolution 2.9Å" /> '''Crystal Structure of ... |
No edit summary |
||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
The crystal structure of NADP-dependent apo-glyceraldehyde-3-phosphate | The crystal structure of NADP-dependent apo-glyceraldehyde-3-phosphate dehydrogenase (apo-GAPDH) from Synechococcus PCC 7942 is reported. The crystal structure was solved by molecular replacement and refined to an R of 21.7% and R(free) of 27.5% at 2.9 angstroms resolution. The structural features of apo-GAPDH are as follows. The S-loop has an extremely flexible conformation and the sulfate ion is only taken into the classical P(i) site. A structural comparison with holo-GAPDHs indicated that the S-loop fixation is essential in the discrimination of NADP and NAD molecules. | ||
==About this Structure== | ==About this Structure== | ||
| Line 10: | Line 10: | ||
==Reference== | ==Reference== | ||
Structure of apo-glyceraldehyde-3-phosphate dehydrogenase from Synechococcus PCC7942., Kitatani T, Nakamura Y, Wada K, Kinoshita T, Tamoi M, Shigeoka S, Tada T, Acta | Structure of apo-glyceraldehyde-3-phosphate dehydrogenase from Synechococcus PCC7942., Kitatani T, Nakamura Y, Wada K, Kinoshita T, Tamoi M, Shigeoka S, Tada T, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):727-30. Epub 2006 Jul 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16880542 16880542] | ||
[[Category: Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating)]] | [[Category: Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 26: | Line 26: | ||
[[Category: rossmann fold]] | [[Category: rossmann fold]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:02:54 2008'' | ||
Revision as of 18:02, 21 February 2008
|
Crystal Structure of apo-form of NADP-Dependent Glyceraldehyde-3-Phosphate Dehydrogenase from Synechococcus Sp.
OverviewOverview
The crystal structure of NADP-dependent apo-glyceraldehyde-3-phosphate dehydrogenase (apo-GAPDH) from Synechococcus PCC 7942 is reported. The crystal structure was solved by molecular replacement and refined to an R of 21.7% and R(free) of 27.5% at 2.9 angstroms resolution. The structural features of apo-GAPDH are as follows. The S-loop has an extremely flexible conformation and the sulfate ion is only taken into the classical P(i) site. A structural comparison with holo-GAPDHs indicated that the S-loop fixation is essential in the discrimination of NADP and NAD molecules.
About this StructureAbout this Structure
2DUU is a Single protein structure of sequence from Synechococcus sp. with as ligand. Active as Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating), with EC number 1.2.1.13 Full crystallographic information is available from OCA.
ReferenceReference
Structure of apo-glyceraldehyde-3-phosphate dehydrogenase from Synechococcus PCC7942., Kitatani T, Nakamura Y, Wada K, Kinoshita T, Tamoi M, Shigeoka S, Tada T, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):727-30. Epub 2006 Jul 29. PMID:16880542
Page seeded by OCA on Thu Feb 21 17:02:54 2008