2dkd: Difference between revisions

Revision as of 17:59, 21 February 2008

Crystal structure of N-acetylglucosamine-phosphate mutase, a member of the alpha-D-phosphohexomutase superfamily, in the product complex

OverviewOverview

N-acetylglucosamine-phosphate mutase (AGM1) is an essential enzyme in the synthetic process of UDP-N-acetylglucosamine (UDP-GlcNAc). UDP-GlcNAc is a UDP sugar that serves as a biosynthetic precursor of glycoproteins, mucopolysaccharides, and the cell wall of bacteria. Thus, a specific inhibitor of AGM1 from pathogenetic fungi could be a new candidate for an antifungal reagent that inhibits cell wall synthesis. AGM1 catalyzes the conversion of N-acetylglucosamine 6-phosphate (GlcNAc-6-P) into N-acetylglucosamine 1-phosphate (GlcNAc-1-P). This enzyme is a member of the alpha-D-phosphohexomutase superfamily, which catalyzes the intramolecular phosphoryl transfer of sugar substrates. Here we report the crystal structures of AGM1 from Candida albicans for the first time, both in the apoform and in the complex forms with the substrate and the product, and discuss its catalytic mechanism. The structure of AGM1 consists of four domains, of which three domains have essentially the same fold. The overall structure is similar to those of phosphohexomutases; however, there are two additional beta-strands in domain 4, and a circular permutation occurs in domain 1. The catalytic cleft is formed by four loops from each domain. The N-acetyl group of the substrate is recognized by Val-370 and Asn-389 in domain 3, from which the substrate specificity arises. By comparing the substrate and product complexes, it is suggested that the substrate rotates about 180 degrees on the axis linking C-4 and the midpoint of the C-5-O-5 bond in the reaction.

About this StructureAbout this Structure

2DKD is a Single protein structure of sequence from Candida albicans with , and as ligands. Active as Phosphoacetylglucosamine mutase, with EC number 5.4.2.3 Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of N-acetylglucosamine-phosphate mutase, a member of the alpha-D-phosphohexomutase superfamily, and its substrate and product complexes., Nishitani Y, Maruyama D, Nonaka T, Kita A, Fukami TA, Mio T, Yamada-Okabe H, Yamada-Okabe T, Miki K, J Biol Chem. 2006 Jul 14;281(28):19740-7. Epub 2006 May 1. PMID:16651269

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-[[Image:2dkd.gif|left|200px]]<br /><applet load="2dkd" size="450" color="white" frame="true" align="right" spinBox="true"
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 caption="2dkd, resolution 2.10&Aring;" />
 '''Crystal structure of N-acetylglucosamine-phosphate mutase, a member of the alpha-D-phosphohexomutase superfamily, in the product complex'''<br />
 ==Overview==
-N-acetylglucosamine-phosphate mutase (AGM1) is an essential enzyme in the, synthetic process of UDP-N-acetylglucosamine (UDP-GlcNAc). UDP-GlcNAc is a, UDP sugar that serves as a biosynthetic precursor of glycoproteins, mucopolysaccharides, and the cell wall of bacteria. Thus, a specific, inhibitor of AGM1 from pathogenetic fungi could be a new candidate for an, antifungal reagent that inhibits cell wall synthesis. AGM1 catalyzes the, conversion of N-acetylglucosamine 6-phosphate (GlcNAc-6-P) into, N-acetylglucosamine 1-phosphate (GlcNAc-1-P). This enzyme is a member of, the alpha-D-phosphohexomutase superfamily, which catalyzes the, intramolecular phosphoryl transfer of sugar substrates. Here we report the, crystal structures of AGM1 from Candida albicans for the first time, both, in the apoform and in the complex forms with the substrate and the, product, and discuss its catalytic mechanism. The structure of AGM1, consists of four domains, of which three domains have essentially the same, fold. The overall structure is similar to those of phosphohexomutases;, however, there are two additional beta-strands in domain 4, and a circular, permutation occurs in domain 1. The catalytic cleft is formed by four, loops from each domain. The N-acetyl group of the substrate is recognized, by Val-370 and Asn-389 in domain 3, from which the substrate specificity, arises. By comparing the substrate and product complexes, it is suggested, that the substrate rotates about 180 degrees on the axis linking C-4 and, the midpoint of the C-5-O-5 bond in the reaction.
+N-acetylglucosamine-phosphate mutase (AGM1) is an essential enzyme in the synthetic process of UDP-N-acetylglucosamine (UDP-GlcNAc). UDP-GlcNAc is a UDP sugar that serves as a biosynthetic precursor of glycoproteins, mucopolysaccharides, and the cell wall of bacteria. Thus, a specific inhibitor of AGM1 from pathogenetic fungi could be a new candidate for an antifungal reagent that inhibits cell wall synthesis. AGM1 catalyzes the conversion of N-acetylglucosamine 6-phosphate (GlcNAc-6-P) into N-acetylglucosamine 1-phosphate (GlcNAc-1-P). This enzyme is a member of the alpha-D-phosphohexomutase superfamily, which catalyzes the intramolecular phosphoryl transfer of sugar substrates. Here we report the crystal structures of AGM1 from Candida albicans for the first time, both in the apoform and in the complex forms with the substrate and the product, and discuss its catalytic mechanism. The structure of AGM1 consists of four domains, of which three domains have essentially the same fold. The overall structure is similar to those of phosphohexomutases; however, there are two additional beta-strands in domain 4, and a circular permutation occurs in domain 1. The catalytic cleft is formed by four loops from each domain. The N-acetyl group of the substrate is recognized by Val-370 and Asn-389 in domain 3, from which the substrate specificity arises. By comparing the substrate and product complexes, it is suggested that the substrate rotates about 180 degrees on the axis linking C-4 and the midpoint of the C-5-O-5 bond in the reaction.
 ==About this Structure==
-DKD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans] with NG1, PO4 and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoacetylglucosamine_mutase Phosphoacetylglucosamine mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.3 5.4.2.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2DKD OCA].
+DKD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans] with <scene name='pdbligand=NG1:'>NG1</scene>, <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoacetylglucosamine_mutase Phosphoacetylglucosamine mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.3 5.4.2.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DKD OCA].
 ==Reference==
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 [[Category: Phosphoacetylglucosamine mutase]]
 [[Category: Single protein]]
-[[Category: Fukami, T.A.]]
+[[Category: Fukami, T A.]]
 [[Category: Kita, A.]]
 [[Category: Maruyama, D.]]
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 [[Category: mutase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:38:34 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:59:38 2008''