2d6f: Difference between revisions
New page: left|200px<br /><applet load="2d6f" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d6f, resolution 3.15Å" /> '''Crystal structure of... |
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[[Image:2d6f.gif|left|200px]]<br /><applet load="2d6f" size=" | [[Image:2d6f.gif|left|200px]]<br /><applet load="2d6f" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2d6f, resolution 3.15Å" /> | caption="2d6f, resolution 3.15Å" /> | ||
'''Crystal structure of Glu-tRNA(Gln) amidotransferase in the complex with tRNA(Gln)'''<br /> | '''Crystal structure of Glu-tRNA(Gln) amidotransferase in the complex with tRNA(Gln)'''<br /> | ||
==Overview== | ==Overview== | ||
Glutaminyl-transfer RNA (Gln-tRNA(Gln)) in archaea is synthesized in a | Glutaminyl-transfer RNA (Gln-tRNA(Gln)) in archaea is synthesized in a pretranslational amidation of misacylated Glu-tRNA(Gln) by the heterodimeric Glu-tRNA(Gln) amidotransferase GatDE. Here we report the crystal structure of the Methanothermobacter thermautotrophicus GatDE complexed to tRNA(Gln) at 3.15 angstroms resolution. Biochemical analysis of GatDE and of tRNA(Gln) mutants characterized the catalytic centers for the enzyme's three reactions (glutaminase, kinase, and amidotransferase activity). A 40 angstrom-long channel for ammonia transport connects the active sites in GatD and GatE. tRNA(Gln) recognition by indirect readout based on shape complementarity of the D loop suggests an early anticodon-independent RNA-based mechanism for adding glutamine to the genetic code. | ||
==About this Structure== | ==About this Structure== | ||
2D6F is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 2D6F is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D6F OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: rna]] | [[Category: rna]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:55:48 2008'' | ||
Revision as of 17:55, 21 February 2008
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Crystal structure of Glu-tRNA(Gln) amidotransferase in the complex with tRNA(Gln)
OverviewOverview
Glutaminyl-transfer RNA (Gln-tRNA(Gln)) in archaea is synthesized in a pretranslational amidation of misacylated Glu-tRNA(Gln) by the heterodimeric Glu-tRNA(Gln) amidotransferase GatDE. Here we report the crystal structure of the Methanothermobacter thermautotrophicus GatDE complexed to tRNA(Gln) at 3.15 angstroms resolution. Biochemical analysis of GatDE and of tRNA(Gln) mutants characterized the catalytic centers for the enzyme's three reactions (glutaminase, kinase, and amidotransferase activity). A 40 angstrom-long channel for ammonia transport connects the active sites in GatD and GatE. tRNA(Gln) recognition by indirect readout based on shape complementarity of the D loop suggests an early anticodon-independent RNA-based mechanism for adding glutamine to the genetic code.
About this StructureAbout this Structure
2D6F is a Protein complex structure of sequences from Methanothermobacter thermautotrophicus with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of RNA-dependent recruitment of glutamine to the genetic code., Oshikane H, Sheppard K, Fukai S, Nakamura Y, Ishitani R, Numata T, Sherrer RL, Feng L, Schmitt E, Panvert M, Blanquet S, Mechulam Y, Soll D, Nureki O, Science. 2006 Jun 30;312(5782):1950-4. PMID:16809540
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