2c4u: Difference between revisions
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==Overview== | ==Overview== | ||
The fluorinase enzyme from Streptomyces cattleya displays an unusual | The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C-F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Delaurentis, W.]] | [[Category: Delaurentis, W.]] | ||
[[Category: Deng, H.]] | [[Category: Deng, H.]] | ||
[[Category: Hagan, D | [[Category: Hagan, D O.]] | ||
[[Category: Mcewan, A | [[Category: Mcewan, A R.]] | ||
[[Category: Mcglinchey, R | [[Category: Mcglinchey, R P.]] | ||
[[Category: Naismith, J | [[Category: Naismith, J H.]] | ||
[[Category: Robinson, D | [[Category: Robinson, D A.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: 5'-fluorodeoxyadanosine synthase]] | [[Category: 5'-fluorodeoxyadanosine synthase]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:44:52 2008'' | ||
Revision as of 17:44, 21 February 2008
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CRYSTAL STRUCTURE OF THE APO FORM OF THE 5'-FLUORO-5'-DEOXYADENOSINE SYNTHASE ENZYME FROM STREPTOMYCES CATTLEYA
OverviewOverview
The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C-F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation.
About this StructureAbout this Structure
2C4U is a Single protein structure of sequence from Streptomyces cattleya with as ligand. Active as Adenosyl-fluoride synthase, with EC number 2.5.1.63 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates., Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA, Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:16604208
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