1oah

File:1oah.gif

spinqualitylabelsall models 1oah, resolution 2.3Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774: THE RELEVANCE OF THE TWO CALCIUM SITES IN THE STRUCTURE OF THE CATALYTIC SUBUNIT (NRFA).

OverviewOverview

The gene encoding cytochrome c nitrite reductase (NrfA) from Desulfovibrio, desulfuricans ATCC 27774 was sequenced and the crystal structure of the, enzyme was determined to 2.3-A resolution. In comparison with homologous, structures, it presents structural differences mainly located at the, regions surrounding the putative substrate inlet and product outlet, and, includes a well defined second calcium site with octahedral geometry, coordinated to propionates of hemes 3 and 4, and caged by a loop, non-existent in the previous structures. The highly negative electrostatic, potential in the environment around hemes 3 and 4 suggests that the main, role of this calcium ion may not be electrostatic but structural, namely, in the stabilization of the conformation of the additional loop that ... [(full description)]

About this StructureAbout this Structure

1OAH is a [Single protein] structure of sequence from [Desulfovibrio desulfuricans] with CA, CL, ZN and HEM as [ligands]. Active as [Nitrite reductase (cytochrome; ammonia-forming)], with EC number [1.7.2.2]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

ReferenceReference

Cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774. The relevance of the two calcium sites in the structure of the catalytic subunit (NrfA)., Cunha CA, Macieira S, Dias JM, Almeida G, Goncalves LL, Costa C, Lampreia J, Huber R, Moura JJ, Moura I, Romao MJ, J Biol Chem. 2003 May 9;278(19):17455-65. Epub 2003 Mar 4. PMID:12618432

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