2bjc: Difference between revisions
New page: left|200px<br /><applet load="2bjc" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bjc" /> '''NMR STRUCTURE OF A PROTEIN-DNA COMPLEX OF AN... |
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'''NMR STRUCTURE OF A PROTEIN-DNA COMPLEX OF AN ALTERED SPECIFICITY MUTANT OF THE LAC REPRESSOR HEADPIECE THAT MIMICS THE GAL REPRESSOR'''<br /> | '''NMR STRUCTURE OF A PROTEIN-DNA COMPLEX OF AN ALTERED SPECIFICITY MUTANT OF THE LAC REPRESSOR HEADPIECE THAT MIMICS THE GAL REPRESSOR'''<br /> | ||
==Overview== | ==Overview== | ||
Recognition of the lac operator by the lac repressor involves specific | Recognition of the lac operator by the lac repressor involves specific interactions between residues in the repressor's recognition helix and bases in the DNA major groove. Tyr17 and Gln18, at positions 1 and 2 in the lac repressor recognition helix, can be exchanged for other amino acids to generate mutant repressors that display altered specificity. We have solved the solution structure of a protein-DNA complex of an altered-specificity mutant lac headpiece in which Tyr17 and Gln18 were exchanged for valine and alanine, respectively, as found in the recognition helix of the gal repressor. As previously described by Lehming et al. (EMBO J. 1987, 6, 3145-3153), this altered-specificity mutant of the lac repressor recognizes a variant lac operator that is similar to the gal operator Oe. The mutant lac headpiece showed the predicted specificity and is also able to mimic the gal repressor by recognizing and bending the natural gal operator Oe. These structural data show that, while most of the anchoring points that help the lac headpiece to assemble on the lac operator were preserved, a different network of protein-DNA interactions connecting Ala17 and Val18 to bases in the DNA major groove drives the specificity towards the altered operator. | ||
==About this Structure== | ==About this Structure== | ||
2BJC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | 2BJC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJC OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Boelens, R.]] | [[Category: Boelens, R.]] | ||
[[Category: Bonvin, A | [[Category: Bonvin, A M.J J.]] | ||
[[Category: Das, D.]] | [[Category: Das, D.]] | ||
[[Category: Folkers, G | [[Category: Folkers, G E.]] | ||
[[Category: Kaptein, R.]] | [[Category: Kaptein, R.]] | ||
[[Category: Salinas, R | [[Category: Salinas, R K.]] | ||
[[Category: altered specificity]] | [[Category: altered specificity]] | ||
[[Category: dna-binding]] | [[Category: dna-binding]] | ||
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[[Category: transcription regulator/dna]] | [[Category: transcription regulator/dna]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:38:28 2008'' | ||
Revision as of 17:38, 21 February 2008
|
NMR STRUCTURE OF A PROTEIN-DNA COMPLEX OF AN ALTERED SPECIFICITY MUTANT OF THE LAC REPRESSOR HEADPIECE THAT MIMICS THE GAL REPRESSOR
OverviewOverview
Recognition of the lac operator by the lac repressor involves specific interactions between residues in the repressor's recognition helix and bases in the DNA major groove. Tyr17 and Gln18, at positions 1 and 2 in the lac repressor recognition helix, can be exchanged for other amino acids to generate mutant repressors that display altered specificity. We have solved the solution structure of a protein-DNA complex of an altered-specificity mutant lac headpiece in which Tyr17 and Gln18 were exchanged for valine and alanine, respectively, as found in the recognition helix of the gal repressor. As previously described by Lehming et al. (EMBO J. 1987, 6, 3145-3153), this altered-specificity mutant of the lac repressor recognizes a variant lac operator that is similar to the gal operator Oe. The mutant lac headpiece showed the predicted specificity and is also able to mimic the gal repressor by recognizing and bending the natural gal operator Oe. These structural data show that, while most of the anchoring points that help the lac headpiece to assemble on the lac operator were preserved, a different network of protein-DNA interactions connecting Ala17 and Val18 to bases in the DNA major groove drives the specificity towards the altered operator.
About this StructureAbout this Structure
2BJC is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Altered specificity in DNA binding by the lac repressor: a mutant lac headpiece that mimics the gal repressor., Kopke Salinas R, Folkers GE, Bonvin AM, Das D, Boelens R, Kaptein R, Chembiochem. 2005 Sep;6(9):1628-37. PMID:16094693
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Escherichia coli
- Protein complex
- Boelens, R.
- Bonvin, A M.J J.
- Das, D.
- Folkers, G E.
- Kaptein, R.
- Salinas, R K.
- Altered specificity
- Dna-binding
- Gal operon
- Gal repressor
- Hth
- Lac headpiece
- Lac operon
- Lac repressor
- Mutant
- Nmr
- Repressor
- Symmetric dimer
- Symmetric dna-binding
- Transcription regulation
- Transcription regulator/dna