2b5n: Difference between revisions
New page: left|200px<br /><applet load="2b5n" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b5n, resolution 2.8Å" /> '''Crystal Structure of ... |
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[[Image:2b5n.gif|left|200px]]<br /><applet load="2b5n" size=" | [[Image:2b5n.gif|left|200px]]<br /><applet load="2b5n" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2b5n, resolution 2.8Å" /> | caption="2b5n, resolution 2.8Å" /> | ||
'''Crystal Structure of the DDB1 BPB Domain'''<br /> | '''Crystal Structure of the DDB1 BPB Domain'''<br /> | ||
==Overview== | ==Overview== | ||
The DDB1-Cul4A ubiquitin ligase complex promotes protein ubiquitination in | The DDB1-Cul4A ubiquitin ligase complex promotes protein ubiquitination in diverse cellular functions and is reprogrammed by the V proteins of paramyxoviruses to degrade STATs and block interferon signaling. Here we report the crystal structures of DDB1 alone and in complex with the simian virus 5 V protein. The DDB1 structure reveals an intertwined three-propeller cluster, which contains two tightly coupled beta propellers with a large pocket in between and a third beta propeller flexibly attached on the side. The rigid double-propeller fold of DDB1 is targeted by the viral V protein, which inserts an entire helix into the double-propeller pocket, whereas the third propeller domain docks DDB1 to the N terminus of the Cul4A scaffold. Together, these results not only provide structural insights into how the virus hijacks the DDB1-Cul4A ubiquitin ligase but also establish a structural framework for understanding the multiple functions of DDB1 in the uniquely assembled cullin-RING E3 machinery. | ||
==About this Structure== | ==About this Structure== | ||
2B5N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with IPA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 2B5N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=IPA:'>IPA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5N OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chen, X.]] | [[Category: Chen, X.]] | ||
[[Category: Garbutt, K | [[Category: Garbutt, K C.]] | ||
[[Category: Li, T.]] | [[Category: Li, T.]] | ||
[[Category: Zheng, N.]] | [[Category: Zheng, N.]] | ||
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[[Category: propeller cluster]] | [[Category: propeller cluster]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:34:26 2008'' | ||
Revision as of 17:34, 21 February 2008
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Crystal Structure of the DDB1 BPB Domain
OverviewOverview
The DDB1-Cul4A ubiquitin ligase complex promotes protein ubiquitination in diverse cellular functions and is reprogrammed by the V proteins of paramyxoviruses to degrade STATs and block interferon signaling. Here we report the crystal structures of DDB1 alone and in complex with the simian virus 5 V protein. The DDB1 structure reveals an intertwined three-propeller cluster, which contains two tightly coupled beta propellers with a large pocket in between and a third beta propeller flexibly attached on the side. The rigid double-propeller fold of DDB1 is targeted by the viral V protein, which inserts an entire helix into the double-propeller pocket, whereas the third propeller domain docks DDB1 to the N terminus of the Cul4A scaffold. Together, these results not only provide structural insights into how the virus hijacks the DDB1-Cul4A ubiquitin ligase but also establish a structural framework for understanding the multiple functions of DDB1 in the uniquely assembled cullin-RING E3 machinery.
About this StructureAbout this Structure
2B5N is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structure of DDB1 in complex with a paramyxovirus V protein: viral hijack of a propeller cluster in ubiquitin ligase., Li T, Chen X, Garbutt KC, Zhou P, Zheng N, Cell. 2006 Jan 13;124(1):105-17. PMID:16413485
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