2b4b: Difference between revisions
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'''SSAT+COA+BE-3-3-3, K6R mutant'''<br /> | '''SSAT+COA+BE-3-3-3, K6R mutant'''<br /> | ||
==Overview== | ==Overview== | ||
Spermidine/spermine N1-acetyltransferase (SSAT) is a key enzyme in the | Spermidine/spermine N1-acetyltransferase (SSAT) is a key enzyme in the control of polyamine levels in human cells, as acetylation of spermidine and spermine triggers export or degradation. Increased intracellular polyamine levels accompany several types of cancers as well as other human diseases, and compounds that affect the expression, activity, or stability of SSAT are being explored as potential therapeutic drugs. We have expressed human SSAT from the cloned cDNA in Escherichia coli and have determined high-resolution structures of wild-type and mutant SSAT, as the free dimer and in binary and ternary complexes with CoA, acetyl-CoA (AcCoA), spermine, and the inhibitor N1,N11bis-(ethyl)-norspermine (BE-3-3-3). These structures show details of binding sites for cofactor, substrates, and inhibitor and provide a framework to understand enzymatic activity, mutations, and the action of potential drugs. Two dimer conformations were observed: a symmetric form with two open surface channels capable of binding substrate or cofactor, and an asymmetric form in which only one of the surface channels appears capable of binding and acetylating polyamines. SSAT was found to self-acetylate lysine-26 in the presence of AcCoA and absence of substrate, a reaction apparently catalzyed by AcCoA bound in the second channel of the asymmetric dimer. These unexpected and intriguing complexities seem likely to have some as yet undefined role in regulating SSAT activity or stability as a part of polyamine homeostasis. Sequence signatures group SSAT with proteins that appear to have thialysine Nepsilon-acetyltransferase activity. | ||
==Disease== | ==Disease== | ||
Known | Known diseases associated with this structure: Keratosis follicularis spinulosa decalvans OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=313020 313020]] | ||
==About this Structure== | ==About this Structure== | ||
2B4B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with COA and B33 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] Full crystallographic information is available from [http:// | 2B4B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=COA:'>COA</scene> and <scene name='pdbligand=B33:'>B33</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B4B OCA]. | ||
==Reference== | ==Reference== | ||
| Line 18: | Line 17: | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bewley, M | [[Category: Bewley, M C.]] | ||
[[Category: Coleman, C | [[Category: Coleman, C S.]] | ||
[[Category: Flanagan, J | [[Category: Flanagan, J M.]] | ||
[[Category: Graziano, V.]] | [[Category: Graziano, V.]] | ||
[[Category: Jiang, J | [[Category: Jiang, J S.]] | ||
[[Category: Matz, E.]] | [[Category: Matz, E.]] | ||
[[Category: NYSGXRC, New | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] | ||
[[Category: Pegg, A | [[Category: Pegg, A P.]] | ||
[[Category: Studier, F | [[Category: Studier, F W.]] | ||
[[Category: B33]] | [[Category: B33]] | ||
[[Category: COA]] | [[Category: COA]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:34:00 2008'' | ||
Revision as of 17:34, 21 February 2008
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SSAT+COA+BE-3-3-3, K6R mutant
OverviewOverview
Spermidine/spermine N1-acetyltransferase (SSAT) is a key enzyme in the control of polyamine levels in human cells, as acetylation of spermidine and spermine triggers export or degradation. Increased intracellular polyamine levels accompany several types of cancers as well as other human diseases, and compounds that affect the expression, activity, or stability of SSAT are being explored as potential therapeutic drugs. We have expressed human SSAT from the cloned cDNA in Escherichia coli and have determined high-resolution structures of wild-type and mutant SSAT, as the free dimer and in binary and ternary complexes with CoA, acetyl-CoA (AcCoA), spermine, and the inhibitor N1,N11bis-(ethyl)-norspermine (BE-3-3-3). These structures show details of binding sites for cofactor, substrates, and inhibitor and provide a framework to understand enzymatic activity, mutations, and the action of potential drugs. Two dimer conformations were observed: a symmetric form with two open surface channels capable of binding substrate or cofactor, and an asymmetric form in which only one of the surface channels appears capable of binding and acetylating polyamines. SSAT was found to self-acetylate lysine-26 in the presence of AcCoA and absence of substrate, a reaction apparently catalzyed by AcCoA bound in the second channel of the asymmetric dimer. These unexpected and intriguing complexities seem likely to have some as yet undefined role in regulating SSAT activity or stability as a part of polyamine homeostasis. Sequence signatures group SSAT with proteins that appear to have thialysine Nepsilon-acetyltransferase activity.
DiseaseDisease
Known diseases associated with this structure: Keratosis follicularis spinulosa decalvans OMIM:[313020]
About this StructureAbout this Structure
2B4B is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Diamine N-acetyltransferase, with EC number 2.3.1.57 Full crystallographic information is available from OCA.
ReferenceReference
Structures of wild-type and mutant human spermidine/spermine N1-acetyltransferase, a potential therapeutic drug target., Bewley MC, Graziano V, Jiang J, Matz E, Studier FW, Pegg AE, Coleman CS, Flanagan JM, Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2063-8. Epub 2006 Feb 2. PMID:16455797
Page seeded by OCA on Thu Feb 21 16:34:00 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Diamine N-acetyltransferase
- Homo sapiens
- Single protein
- Bewley, M C.
- Coleman, C S.
- Flanagan, J M.
- Graziano, V.
- Jiang, J S.
- Matz, E.
- NYSGXRC, New York Structural GenomiX Research Consortium.
- Pegg, A P.
- Studier, F W.
- B33
- COA
- New york structural genomix research consortium
- Nysgxrc
- Protein structure initiative
- Psi
- Structural genomics