2b1a: Difference between revisions
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caption="2b1a, resolution 2.348Å" /> | caption="2b1a, resolution 2.348Å" /> | ||
'''Crystal structure analysis of anti-HIV-1 V3 Fab 2219 in complex with UG1033 peptide'''<br /> | '''Crystal structure analysis of anti-HIV-1 V3 Fab 2219 in complex with UG1033 peptide'''<br /> | ||
==Overview== | ==Overview== | ||
Human monoclonal antibody 2219 is a neutralizing antibody isolated from a | Human monoclonal antibody 2219 is a neutralizing antibody isolated from a human immunodeficiency virus type 1-infected individual. 2219 was originally selected for binding to a V3 fusion protein and can neutralize primary isolates from subtypes B, A, and F. Thus, 2219 represents a cross-reactive, human anti-V3 antibody. Fab 2219 binds to one face of the variable V3 beta-hairpin, primarily contacting conserved residues on the N-terminal beta-strand of V3, leaving the V3 crown or tip largely accessible. Three V3/2219 complexes reveal the antibody-bound conformations for both the N- and C-terminal regions that flank the V3 crown and illustrate how twisting of the V3 loop alters the relative dispositions and pairing of the amino acids in the adjacent V3 beta-strands and how the antibody can accommodate V3 loops with different sequences. | ||
==About this Structure== | ==About this Structure== | ||
2B1A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | 2B1A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B1A OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Gorny, M | [[Category: Gorny, M K.]] | ||
[[Category: Stanfield, R | [[Category: Stanfield, R L.]] | ||
[[Category: Wilson, I | [[Category: Wilson, I A.]] | ||
[[Category: Zolla-Pazner, S.]] | [[Category: Zolla-Pazner, S.]] | ||
[[Category: fab-peptide complex; hiv-1; gp120; v3 loop]] | [[Category: fab-peptide complex; hiv-1; gp120; v3 loop]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:33:07 2008'' | ||
Revision as of 17:33, 21 February 2008
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Crystal structure analysis of anti-HIV-1 V3 Fab 2219 in complex with UG1033 peptide
OverviewOverview
Human monoclonal antibody 2219 is a neutralizing antibody isolated from a human immunodeficiency virus type 1-infected individual. 2219 was originally selected for binding to a V3 fusion protein and can neutralize primary isolates from subtypes B, A, and F. Thus, 2219 represents a cross-reactive, human anti-V3 antibody. Fab 2219 binds to one face of the variable V3 beta-hairpin, primarily contacting conserved residues on the N-terminal beta-strand of V3, leaving the V3 crown or tip largely accessible. Three V3/2219 complexes reveal the antibody-bound conformations for both the N- and C-terminal regions that flank the V3 crown and illustrate how twisting of the V3 loop alters the relative dispositions and pairing of the amino acids in the adjacent V3 beta-strands and how the antibody can accommodate V3 loops with different sequences.
About this StructureAbout this Structure
2B1A is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of human immunodeficiency virus type 1 (HIV-1) neutralizing antibody 2219 in complex with three different V3 peptides reveal a new binding mode for HIV-1 cross-reactivity., Stanfield RL, Gorny MK, Zolla-Pazner S, Wilson IA, J Virol. 2006 Jun;80(12):6093-105. PMID:16731948
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