2azx: Difference between revisions
New page: left|200px<br /> <applet load="2azx" size="450" color="white" frame="true" align="right" spinBox="true" caption="2azx, resolution 2.8Å" /> '''Charged and uncharge... |
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[[Image:2azx.gif|left|200px]]<br /> | [[Image:2azx.gif|left|200px]]<br /><applet load="2azx" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2azx" size=" | |||
caption="2azx, resolution 2.8Å" /> | caption="2azx, resolution 2.8Å" /> | ||
'''Charged and uncharged tRNAs adopt distinct conformations when complexed with human tryptophanyl-tRNA synthetase'''<br /> | '''Charged and uncharged tRNAs adopt distinct conformations when complexed with human tryptophanyl-tRNA synthetase'''<br /> | ||
==Overview== | ==Overview== | ||
Aminoacylation of tRNA is the first step of protein synthesis. Here, we | Aminoacylation of tRNA is the first step of protein synthesis. Here, we report the co-crystal structure of human tryptophanyl-tRNA synthetase and tRNATrp. This enzyme is reported to interact directly with elongation factor 1alpha, which carries charged tRNA to the ribosome. Crystals were generated from a 50/50% mixture of charged and uncharged tRNATrp. These crystals captured two conformations of the complex, which are nearly identical with respect to the protein and a bound tryptophan. They are distinguished by the way tRNA is bound. In one, uncharged tRNA is bound across the dimer, with anticodon and acceptor stem interacting with separate subunits. In this cross-dimer tRNA complex, the class I enzyme has a class II-like tRNA binding mode. This structure accounts for biochemical investigations of human TrpRS, including species-specific charging. In the other conformation, presumptive aminoacylated tRNA is bound only by the anticodon, the acceptor stem being free and having space to interact precisely with EF-1alpha, suggesting that the product of aminoacylation can be directly handed off to EF-1alpha for the next step of protein synthesis. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
2AZX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4, MG, TRP and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Tryptophan--tRNA_ligase Tryptophan--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.2 6.1.1.2] Full crystallographic information is available from [http:// | 2AZX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=TRP:'>TRP</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Tryptophan--tRNA_ligase Tryptophan--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.2 6.1.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZX OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Tryptophan--tRNA ligase]] | [[Category: Tryptophan--tRNA ligase]] | ||
[[Category: Ewalt, K | [[Category: Ewalt, K L.]] | ||
[[Category: Kohrer, C.]] | [[Category: Kohrer, C.]] | ||
[[Category: Liu, J.]] | [[Category: Liu, J.]] | ||
[[Category: McRee, D | [[Category: McRee, D E.]] | ||
[[Category: Otero, F | [[Category: Otero, F J.]] | ||
[[Category: RajBhandary, U | [[Category: RajBhandary, U L.]] | ||
[[Category: Schimmel, P.]] | [[Category: Schimmel, P.]] | ||
[[Category: Skene, R | [[Category: Skene, R J.]] | ||
[[Category: Swairjo, M | [[Category: Swairjo, M A.]] | ||
[[Category: Yang, X | [[Category: Yang, X L.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: two synthetase-trna complex with distinct conformations]] | [[Category: two synthetase-trna complex with distinct conformations]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:32:47 2008'' | ||
Revision as of 17:32, 21 February 2008
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Charged and uncharged tRNAs adopt distinct conformations when complexed with human tryptophanyl-tRNA synthetase
OverviewOverview
Aminoacylation of tRNA is the first step of protein synthesis. Here, we report the co-crystal structure of human tryptophanyl-tRNA synthetase and tRNATrp. This enzyme is reported to interact directly with elongation factor 1alpha, which carries charged tRNA to the ribosome. Crystals were generated from a 50/50% mixture of charged and uncharged tRNATrp. These crystals captured two conformations of the complex, which are nearly identical with respect to the protein and a bound tryptophan. They are distinguished by the way tRNA is bound. In one, uncharged tRNA is bound across the dimer, with anticodon and acceptor stem interacting with separate subunits. In this cross-dimer tRNA complex, the class I enzyme has a class II-like tRNA binding mode. This structure accounts for biochemical investigations of human TrpRS, including species-specific charging. In the other conformation, presumptive aminoacylated tRNA is bound only by the anticodon, the acceptor stem being free and having space to interact precisely with EF-1alpha, suggesting that the product of aminoacylation can be directly handed off to EF-1alpha for the next step of protein synthesis.
DiseaseDisease
Known disease associated with this structure: Wolcott-Rallison syndrome OMIM:[604032]
About this StructureAbout this Structure
2AZX is a Single protein structure of sequence from Homo sapiens with , , and as ligands. Active as Tryptophan--tRNA ligase, with EC number 6.1.1.2 Full crystallographic information is available from OCA.
ReferenceReference
Two conformations of a crystalline human tRNA synthetase-tRNA complex: implications for protein synthesis., Yang XL, Otero FJ, Ewalt KL, Liu J, Swairjo MA, Kohrer C, RajBhandary UL, Skene RJ, McRee DE, Schimmel P, EMBO J. 2006 Jun 21;25(12):2919-29. Epub 2006 May 25. PMID:16724112
Page seeded by OCA on Thu Feb 21 16:32:47 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Homo sapiens
- Single protein
- Tryptophan--tRNA ligase
- Ewalt, K L.
- Kohrer, C.
- Liu, J.
- McRee, D E.
- Otero, F J.
- RajBhandary, U L.
- Schimmel, P.
- Skene, R J.
- Swairjo, M A.
- Yang, X L.
- GOL
- MG
- SO4
- TRP
- Association complex and dissociation complex
- Two synthetase-trna complex with distinct conformations