2axc: Difference between revisions
New page: left|200px<br /><applet load="2axc" size="450" color="white" frame="true" align="right" spinBox="true" caption="2axc, resolution 1.70Å" /> '''Crystal structure of... |
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[[Image:2axc.gif|left|200px]]<br /><applet load="2axc" size=" | [[Image:2axc.gif|left|200px]]<br /><applet load="2axc" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2axc, resolution 1.70Å" /> | caption="2axc, resolution 1.70Å" /> | ||
'''Crystal structure of ColE7 translocation domain'''<br /> | '''Crystal structure of ColE7 translocation domain'''<br /> | ||
==Overview== | ==Overview== | ||
ColE7 is a nuclease-type colicin released from Escherichia coli to kill | ColE7 is a nuclease-type colicin released from Escherichia coli to kill sensitive bacterial cells by degrading the nucleic acid molecules in their cytoplasm. ColE7 is classified as one of the group A colicins, since the N-terminal translocation domain (T-domain) of the nuclease-type colicins interact with specific membrane-bound or periplasmic Tol proteins during protein import. Here, we show that if the N-terminal tail of ColE7 is deleted, ColE7 (residues 63-576) loses its bactericidal activity against E.coli. Moreover, TolB protein interacts directly with the T-domain of ColE7 (residues 1-316), but not with the N-terminal deleted T-domain (residues 60-316), as detected by co-immunoprecipitation experiments, confirming that the N-terminal tail is required for ColE7 interactions with TolB. The crystal structure of the N-terminal tail deleted ColE7 T-domain was determined by the multi-wavelength anomalous dispersion method at a resolution of 1.7 angstroms. The structure of the ColE7 T-domain superimposes well with the T-domain of ColE3 and TR-domain of ColB, a group A Tol-dependent colicin and a group B TonB-dependent colicin, respectively. The structural resemblance of group A and B colicins implies that the two groups of colicins may share a mechanistic connection during cellular import. | ||
==About this Structure== | ==About this Structure== | ||
2AXC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2AXC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AXC OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chak, K | [[Category: Chak, K F.]] | ||
[[Category: Cheng, Y | [[Category: Cheng, Y S.]] | ||
[[Category: Doudeva, L | [[Category: Doudeva, L G.]] | ||
[[Category: Shi, Z.]] | [[Category: Shi, Z.]] | ||
[[Category: Yang, W | [[Category: Yang, W Z.]] | ||
[[Category: Yuan, H | [[Category: Yuan, H S.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: translocation domain]] | [[Category: translocation domain]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:31:59 2008'' | ||
Revision as of 17:31, 21 February 2008
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Crystal structure of ColE7 translocation domain
OverviewOverview
ColE7 is a nuclease-type colicin released from Escherichia coli to kill sensitive bacterial cells by degrading the nucleic acid molecules in their cytoplasm. ColE7 is classified as one of the group A colicins, since the N-terminal translocation domain (T-domain) of the nuclease-type colicins interact with specific membrane-bound or periplasmic Tol proteins during protein import. Here, we show that if the N-terminal tail of ColE7 is deleted, ColE7 (residues 63-576) loses its bactericidal activity against E.coli. Moreover, TolB protein interacts directly with the T-domain of ColE7 (residues 1-316), but not with the N-terminal deleted T-domain (residues 60-316), as detected by co-immunoprecipitation experiments, confirming that the N-terminal tail is required for ColE7 interactions with TolB. The crystal structure of the N-terminal tail deleted ColE7 T-domain was determined by the multi-wavelength anomalous dispersion method at a resolution of 1.7 angstroms. The structure of the ColE7 T-domain superimposes well with the T-domain of ColE3 and TR-domain of ColB, a group A Tol-dependent colicin and a group B TonB-dependent colicin, respectively. The structural resemblance of group A and B colicins implies that the two groups of colicins may share a mechanistic connection during cellular import.
About this StructureAbout this Structure
2AXC is a Single protein structure of sequence from Escherichia coli with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
High-resolution crystal structure of a truncated ColE7 translocation domain: implications for colicin transport across membranes., Cheng YS, Shi Z, Doudeva LG, Yang WZ, Chak KF, Yuan HS, J Mol Biol. 2006 Feb 10;356(1):22-31. Epub 2005 Dec 5. PMID:16360169
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