2atf: Difference between revisions
New page: left|200px<br /><applet load="2atf" size="450" color="white" frame="true" align="right" spinBox="true" caption="2atf, resolution 1.75Å" /> '''X-RAY STRUCTURE OF c... |
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[[Image:2atf.gif|left|200px]]<br /><applet load="2atf" size=" | [[Image:2atf.gif|left|200px]]<br /><applet load="2atf" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2atf, resolution 1.75Å" /> | caption="2atf, resolution 1.75Å" /> | ||
'''X-RAY STRUCTURE OF cysteine dioxygenase type I FROM MUS MUSCULUS MM.241056'''<br /> | '''X-RAY STRUCTURE OF cysteine dioxygenase type I FROM MUS MUSCULUS MM.241056'''<br /> | ||
==Overview== | ==Overview== | ||
Cysteine dioxygenase (CDO) catalyzes the oxidation of l-cysteine to | Cysteine dioxygenase (CDO) catalyzes the oxidation of l-cysteine to cysteine sulfinic acid. Deficiencies in this enzyme have been linked to autoimmune diseases and neurological disorders. The x-ray crystal structure of CDO from Mus musculus was solved to a nominal resolution of 1.75 Angstroms. The sequence is 91% identical to that of a human homolog. The structure reveals that CDO adopts the typical beta-barrel fold of the cupin superfamily. The NE2 atoms of His-86, -88, and -140 provide the metal binding site. The structure further revealed a covalent linkage between the side chains of Cys-93 and Tyr-157, the cysteine of which is conserved only in eukaryotic proteins. Metal analysis showed that the recombinant enzyme contained a mixture of iron, nickel, and zinc, with increased iron content associated with increased catalytic activity. Details of the predicted active site are used to present and discuss a plausible mechanism of action for the enzyme. | ||
==About this Structure== | ==About this Structure== | ||
2ATF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NI and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cysteine_dioxygenase Cysteine dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.20 1.13.11.20] Full crystallographic information is available from [http:// | 2ATF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NI:'>NI</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cysteine_dioxygenase Cysteine dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.20 1.13.11.20] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ATF OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Allard, S | [[Category: Allard, S T.M.]] | ||
[[Category: Bingman, C | [[Category: Bingman, C A.]] | ||
[[Category: Bitto, E.]] | [[Category: Bitto, E.]] | ||
[[Category: CESG, Center | [[Category: CESG, Center for Eukaryotic Structural Genomics.]] | ||
[[Category: Jr., G | [[Category: Jr., G N.Phillips.]] | ||
[[Category: Mccoy, J | [[Category: Mccoy, J G.]] | ||
[[Category: Wesenberg, G | [[Category: Wesenberg, G E.]] | ||
[[Category: EDO]] | [[Category: EDO]] | ||
[[Category: NI]] | [[Category: NI]] | ||
| Line 27: | Line 27: | ||
[[Category: cesg]] | [[Category: cesg]] | ||
[[Category: cupin family]] | [[Category: cupin family]] | ||
[[Category: mm | [[Category: mm 241056]] | ||
[[Category: pfam05995 | [[Category: pfam05995 2 cdo_i]] | ||
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi]] | [[Category: psi]] | ||
[[Category: structural genomics]] | [[Category: structural genomics]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:30:49 2008'' | ||
Revision as of 17:30, 21 February 2008
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X-RAY STRUCTURE OF cysteine dioxygenase type I FROM MUS MUSCULUS MM.241056
OverviewOverview
Cysteine dioxygenase (CDO) catalyzes the oxidation of l-cysteine to cysteine sulfinic acid. Deficiencies in this enzyme have been linked to autoimmune diseases and neurological disorders. The x-ray crystal structure of CDO from Mus musculus was solved to a nominal resolution of 1.75 Angstroms. The sequence is 91% identical to that of a human homolog. The structure reveals that CDO adopts the typical beta-barrel fold of the cupin superfamily. The NE2 atoms of His-86, -88, and -140 provide the metal binding site. The structure further revealed a covalent linkage between the side chains of Cys-93 and Tyr-157, the cysteine of which is conserved only in eukaryotic proteins. Metal analysis showed that the recombinant enzyme contained a mixture of iron, nickel, and zinc, with increased iron content associated with increased catalytic activity. Details of the predicted active site are used to present and discuss a plausible mechanism of action for the enzyme.
About this StructureAbout this Structure
2ATF is a Single protein structure of sequence from Mus musculus with and as ligands. Active as Cysteine dioxygenase, with EC number 1.13.11.20 Full crystallographic information is available from OCA.
ReferenceReference
Structure and mechanism of mouse cysteine dioxygenase., McCoy JG, Bailey LJ, Bitto E, Bingman CA, Aceti DJ, Fox BG, Phillips GN Jr, Proc Natl Acad Sci U S A. 2006 Feb 28;103(9):3084-9. Epub 2006 Feb 21. PMID:16492780
Page seeded by OCA on Thu Feb 21 16:30:49 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Cysteine dioxygenase
- Mus musculus
- Single protein
- Allard, S T.M.
- Bingman, C A.
- Bitto, E.
- CESG, Center for Eukaryotic Structural Genomics.
- Jr., G N.Phillips.
- Mccoy, J G.
- Wesenberg, G E.
- EDO
- NI
- Bc013638
- Center for eukaryotic structural genomics
- Cesg
- Cupin family
- Mm 241056
- Pfam05995 2 cdo i
- Protein structure initiative
- Psi
- Structural genomics