2alx: Difference between revisions

Revision as of 17:28, 21 February 2008

Ribonucleotide Reductase R2 from Escherichia coli in space group P6(1)22

OverviewOverview

A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6(1)22 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl(2) or MnCl(2) results in full occupancy of the dinuclear metal site. The structure of the Mn(II)-loaded form was determined to 2.6 Angstroms resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms.

About this StructureAbout this Structure

2ALX is a Single protein structure of sequence from Escherichia coli with and as ligands. Active as Ribonucleoside-diphosphate reductase, with EC number 1.17.4.1 Full crystallographic information is available from OCA.

ReferenceReference

Structure of Escherichia coli ribonucleotide reductase R2 in space group P6122., Sommerhalter M, Saleh L, Bollinger JM Jr, Rosenzweig AC, Acta Crystallogr D Biol Crystallogr. 2005 Dec;61(Pt 12):1649-54. Epub 2005, Nov 19. PMID:16301799

Page seeded by OCA on Thu Feb 21 16:28:41 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:2alx.gif|left|200px]]<br /><applet load="2alx" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2alx.gif|left|200px]]<br /><applet load="2alx" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2alx, resolution 2.600&Aring;" />
 '''Ribonucleotide Reductase R2 from Escherichia coli in space group P6(1)22'''<br />
 ==Overview==
-A new crystal form of wild-type ribonucleotide reductase R2 from, Escherichia coli was obtained. Crystals grow in space group P6(1)22 with, one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry, axis generates the physiological dimeric form of R2. Co-crystallization, with CoCl(2) or MnCl(2) results in full occupancy of the dinuclear metal, site. The structure of the Mn(II)-loaded form was determined to 2.6, Angstroms resolution by molecular replacement. The crystallization, conditions, backbone conformation, crystal-packing interactions and metal, centers are compared with those of previously determined crystal forms.
+A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6(1)22 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl(2) or MnCl(2) results in full occupancy of the dinuclear metal site. The structure of the Mn(II)-loaded form was determined to 2.6 Angstroms resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms.
 ==About this Structure==
-ALX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MN and HG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ALX OCA].
+ALX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=HG:'>HG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ALX OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Ribonucleoside-diphosphate reductase]]
 [[Category: Single protein]]
-[[Category: Jr., J.M.Bollinger.]]
+[[Category: Jr., J M.Bollinger.]]
-[[Category: Rosenzweig, A.C.]]
+[[Category: Rosenzweig, A C.]]
 [[Category: Saleh, L.]]
 [[Category: Sommerhalter, M.]]
@@ Line 25: / Line 25: @@
 [[Category: ribonucleotide reductase r2]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:12:48 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:28:41 2008''