2adc: Difference between revisions
New page: left|200px<br /> <applet load="2adc" size="450" color="white" frame="true" align="right" spinBox="true" caption="2adc" /> '''Solution structure of Polypyrimidine Tract ... |
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'''Solution structure of Polypyrimidine Tract Binding protein RBD34 complexed with CUCUCU RNA'''<br /> | '''Solution structure of Polypyrimidine Tract Binding protein RBD34 complexed with CUCUCU RNA'''<br /> | ||
==Overview== | ==Overview== | ||
The polypyrimidine tract binding protein (PTB) is a 58-kilodalton RNA | The polypyrimidine tract binding protein (PTB) is a 58-kilodalton RNA binding protein involved in multiple aspects of messenger RNA metabolism, including the repression of alternative exons. We have determined the solution structures of the four RNA binding domains (RBDs) of PTB, each bound to a CUCUCU oligonucleotide. Each RBD binds RNA with a different binding specificity. RBD3 and RBD4 interact, resulting in an antiparallel orientation of their bound RNAs. Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor. | ||
==About this Structure== | ==About this Structure== | ||
2ADC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | 2ADC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADC OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Allain, F | [[Category: Allain, F H.T.]] | ||
[[Category: Auweter, S | [[Category: Auweter, S D.]] | ||
[[Category: Black, D | [[Category: Black, D L.]] | ||
[[Category: Erat, M.]] | [[Category: Erat, M.]] | ||
[[Category: Hargous, Y.]] | [[Category: Hargous, Y.]] | ||
[[Category: Henning, A.]] | [[Category: Henning, A.]] | ||
[[Category: Oberstrass, F | [[Category: Oberstrass, F C.]] | ||
[[Category: Pitsch, S.]] | [[Category: Pitsch, S.]] | ||
[[Category: Reymond, L.]] | [[Category: Reymond, L.]] | ||
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[[Category: rrm]] | [[Category: rrm]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:26:14 2008'' | ||
Revision as of 17:26, 21 February 2008
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Solution structure of Polypyrimidine Tract Binding protein RBD34 complexed with CUCUCU RNA
OverviewOverview
The polypyrimidine tract binding protein (PTB) is a 58-kilodalton RNA binding protein involved in multiple aspects of messenger RNA metabolism, including the repression of alternative exons. We have determined the solution structures of the four RNA binding domains (RBDs) of PTB, each bound to a CUCUCU oligonucleotide. Each RBD binds RNA with a different binding specificity. RBD3 and RBD4 interact, resulting in an antiparallel orientation of their bound RNAs. Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor.
About this StructureAbout this Structure
2ADC is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Structure of PTB bound to RNA: specific binding and implications for splicing regulation., Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH, Science. 2005 Sep 23;309(5743):2054-7. PMID:16179478
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