2ab2: Difference between revisions
New page: left|200px<br /> <applet load="2ab2" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ab2, resolution 1.850Å" /> '''Mineralocorticoid ... |
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[[Image:2ab2.gif|left|200px]]<br /> | [[Image:2ab2.gif|left|200px]]<br /><applet load="2ab2" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="2ab2, resolution 1.850Å" /> | caption="2ab2, resolution 1.850Å" /> | ||
'''Mineralocorticoid Receptor Double Mutant with Bound Spironolactone'''<br /> | '''Mineralocorticoid Receptor Double Mutant with Bound Spironolactone'''<br /> | ||
==Overview== | ==Overview== | ||
Ligand binding is the first step in hormone regulation of | Ligand binding is the first step in hormone regulation of mineralocorticoid receptor (MR) activity. Here, we report multiple crystal structures of MR (NR3C2) bound to both agonist and antagonists. These structures combined with mutagenesis studies reveal that maximal receptor activation involves an intricate ligand-mediated hydrogen bond network with Asn770 which serves dual roles: stabilization of the loop preceding the C-terminal activation function-2 helix and direct contact with the hormone ligand. In addition, most activating ligands hydrogen bond to Thr945 on helix 10. Structural characterization of the naturally occurring S810L mutant explains how stabilization of a helix 3/helix 5 interaction can circumvent the requirement for this hydrogen bond network. Taken together, these results explain the potency of MR activation by aldosterone, the weak activation induced by progesterone and the antihypertensive agent spironolactone, and the binding selectivity of cortisol over cortisone. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
2AB2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and SNL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2AB2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=SNL:'>SNL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB2 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Apolito, C | [[Category: Apolito, C J.]] | ||
[[Category: Bledsoe, R | [[Category: Bledsoe, R K.]] | ||
[[Category: Holt, J | [[Category: Holt, J A.]] | ||
[[Category: Lambert, M | [[Category: Lambert, M H.]] | ||
[[Category: Madauss, K | [[Category: Madauss, K P.]] | ||
[[Category: Pearce, K | [[Category: Pearce, K H.]] | ||
[[Category: Stanley, T | [[Category: Stanley, T B.]] | ||
[[Category: Stewart, E | [[Category: Stewart, E L.]] | ||
[[Category: Trump, R | [[Category: Trump, R P.]] | ||
[[Category: Williams, S | [[Category: Williams, S P.]] | ||
[[Category: Willson, T | [[Category: Willson, T M.]] | ||
[[Category: SNL]] | [[Category: SNL]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: steroid receptor]] | [[Category: steroid receptor]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:25:44 2008'' | ||
Revision as of 17:25, 21 February 2008
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Mineralocorticoid Receptor Double Mutant with Bound Spironolactone
OverviewOverview
Ligand binding is the first step in hormone regulation of mineralocorticoid receptor (MR) activity. Here, we report multiple crystal structures of MR (NR3C2) bound to both agonist and antagonists. These structures combined with mutagenesis studies reveal that maximal receptor activation involves an intricate ligand-mediated hydrogen bond network with Asn770 which serves dual roles: stabilization of the loop preceding the C-terminal activation function-2 helix and direct contact with the hormone ligand. In addition, most activating ligands hydrogen bond to Thr945 on helix 10. Structural characterization of the naturally occurring S810L mutant explains how stabilization of a helix 3/helix 5 interaction can circumvent the requirement for this hydrogen bond network. Taken together, these results explain the potency of MR activation by aldosterone, the weak activation induced by progesterone and the antihypertensive agent spironolactone, and the binding selectivity of cortisol over cortisone.
DiseaseDisease
Known diseases associated with this structure: Hypertension, early-onset, autosomal dominant, with exacerbation in pregnancy OMIM:[600983], Pseudohypoaldosteronism type I, autosomal dominant OMIM:[600983]
About this StructureAbout this Structure
2AB2 is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
A ligand-mediated hydrogen bond network required for the activation of the mineralocorticoid receptor., Bledsoe RK, Madauss KP, Holt JA, Apolito CJ, Lambert MH, Pearce KH, Stanley TB, Stewart EL, Trump RP, Willson TM, Williams SP, J Biol Chem. 2005 Sep 2;280(35):31283-93. Epub 2005 Jun 20. PMID:15967794
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