2a79: Difference between revisions

Revision as of 17:24, 21 February 2008

Mammalian Shaker Kv1.2 potassium channel- beta subunit complex

OverviewOverview

Voltage-dependent potassium ion (K+) channels (Kv channels) conduct K+ ions across the cell membrane in response to changes in the membrane voltage, thereby regulating neuronal excitability by modulating the shape and frequency of action potentials. Here we report the crystal structure, at a resolution of 2.9 angstroms, of a mammalian Kv channel, Kv1.2, which is a member of the Shaker K+ channel family. This structure is in complex with an oxido-reductase beta subunit of the kind that can regulate mammalian Kv channels in their native cell environment. The activation gate of the pore is open. Large side portals communicate between the pore and the cytoplasm. Electrostatic properties of the side portals and positions of the T1 domain and beta subunit are consistent with electrophysiological studies of inactivation gating and with the possibility of K+ channel regulation by the beta subunit.

About this StructureAbout this Structure

2A79 is a Protein complex structure of sequences from Rattus norvegicus with and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a mammalian voltage-dependent Shaker family K+ channel., Long SB, Campbell EB, Mackinnon R, Science. 2005 Aug 5;309(5736):897-903. Epub 2005 Jul 7. PMID:16002581

Page seeded by OCA on Thu Feb 21 16:24:16 2008

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OCA

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-[[Image:2a79.gif|left|200px]]<br /><applet load="2a79" size="450" color="white" frame="true" align="right" spinBox="true"
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 caption="2a79, resolution 2.90&Aring;" />
 '''Mammalian Shaker Kv1.2 potassium channel- beta subunit complex'''<br />
 ==Overview==
-Voltage-dependent potassium ion (K+) channels (Kv channels) conduct K+, ions across the cell membrane in response to changes in the membrane, voltage, thereby regulating neuronal excitability by modulating the shape, and frequency of action potentials. Here we report the crystal structure, at a resolution of 2.9 angstroms, of a mammalian Kv channel, Kv1.2, which, is a member of the Shaker K+ channel family. This structure is in complex, with an oxido-reductase beta subunit of the kind that can regulate, mammalian Kv channels in their native cell environment. The activation, gate of the pore is open. Large side portals communicate between the pore, and the cytoplasm. Electrostatic properties of the side portals and, positions of the T1 domain and beta subunit are consistent with, electrophysiological studies of inactivation gating and with the, possibility of K+ channel regulation by the beta subunit.
+Voltage-dependent potassium ion (K+) channels (Kv channels) conduct K+ ions across the cell membrane in response to changes in the membrane voltage, thereby regulating neuronal excitability by modulating the shape and frequency of action potentials. Here we report the crystal structure, at a resolution of 2.9 angstroms, of a mammalian Kv channel, Kv1.2, which is a member of the Shaker K+ channel family. This structure is in complex with an oxido-reductase beta subunit of the kind that can regulate mammalian Kv channels in their native cell environment. The activation gate of the pore is open. Large side portals communicate between the pore and the cytoplasm. Electrostatic properties of the side portals and positions of the T1 domain and beta subunit are consistent with electrophysiological studies of inactivation gating and with the possibility of K+ channel regulation by the beta subunit.
 ==About this Structure==
-A79 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with K and NAP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A79 OCA].
+A79 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=K:'>K</scene> and <scene name='pdbligand=NAP:'>NAP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A79 OCA].
 ==Reference==
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 [[Category: Protein complex]]
 [[Category: Rattus norvegicus]]
-[[Category: Campbell, E.B.]]
+[[Category: Campbell, E B.]]
-[[Category: Long, S.B.]]
+[[Category: Long, S B.]]
 [[Category: MacKinnon, R.]]
 [[Category: K]]
@@ Line 20: / Line 20: @@
 [[Category: eukaryotic]]
 [[Category: ion channel]]
-[[Category: kv1.2]]
+[[Category: kv1 2]]
 [[Category: membrane protein]]
 [[Category: potassium channel]]
@@ Line 27: / Line 27: @@
 [[Category: voltage sensor]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:57:30 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:24:16 2008''