2a5f: Difference between revisions

Revision as of 17:23, 21 February 2008

Cholera toxin A1 subunit bound to its substrate, NAD+, and its human protein activator, ARF6

OverviewOverview

The Vibrio cholerae bacterium causes devastating diarrhea when it infects the human intestine. The key event is adenosine diphosphate (ADP)-ribosylation of the human signaling protein GSalpha, catalyzed by the cholera toxin A1 subunit (CTA1). This reaction is allosterically activated by human ADP-ribosylation factors (ARFs), a family of essential and ubiquitous G proteins. Crystal structures of a CTA1:ARF6-GTP (guanosine triphosphate) complex reveal that binding of the human activator elicits dramatic changes in CTA1 loop regions that allow nicotinamide adenine dinucleotide (NAD+) to bind to the active site. The extensive toxin:ARF-GTP interface surface mimics ARF-GTP recognition of normal cellular protein partners, which suggests that the toxin has evolved to exploit promiscuous binding properties of ARFs.

About this StructureAbout this Structure

2A5F is a Protein complex structure of sequences from Homo sapiens and Vibrio cholerae with , , , and as ligands. Active as NAD(+)--diphthamide ADP-ribosyltransferase, with EC number 2.4.2.36 Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:16099990

Page seeded by OCA on Thu Feb 21 16:23:46 2008

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OCA

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-[[Image:2a5f.gif|left|200px]]<br />
+[[Image:2a5f.gif|left|200px]]<br /><applet load="2a5f" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="2a5f" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2a5f, resolution 2.02&Aring;" />
 '''Cholera toxin A1 subunit bound to its substrate, NAD+, and its human protein activator, ARF6'''<br />
 ==Overview==
-The Vibrio cholerae bacterium causes devastating diarrhea when it infects, the human intestine. The key event is adenosine diphosphate, (ADP)-ribosylation of the human signaling protein GSalpha, catalyzed by, the cholera toxin A1 subunit (CTA1). This reaction is allosterically, activated by human ADP-ribosylation factors (ARFs), a family of essential, and ubiquitous G proteins. Crystal structures of a CTA1:ARF6-GTP, (guanosine triphosphate) complex reveal that binding of the human, activator elicits dramatic changes in CTA1 loop regions that allow, nicotinamide adenine dinucleotide (NAD+) to bind to the active site. The, extensive toxin:ARF-GTP interface surface mimics ARF-GTP recognition of, normal cellular protein partners, which suggests that the toxin has, evolved to exploit promiscuous binding properties of ARFs.
+The Vibrio cholerae bacterium causes devastating diarrhea when it infects the human intestine. The key event is adenosine diphosphate (ADP)-ribosylation of the human signaling protein GSalpha, catalyzed by the cholera toxin A1 subunit (CTA1). This reaction is allosterically activated by human ADP-ribosylation factors (ARFs), a family of essential and ubiquitous G proteins. Crystal structures of a CTA1:ARF6-GTP (guanosine triphosphate) complex reveal that binding of the human activator elicits dramatic changes in CTA1 loop regions that allow nicotinamide adenine dinucleotide (NAD+) to bind to the active site. The extensive toxin:ARF-GTP interface surface mimics ARF-GTP recognition of normal cellular protein partners, which suggests that the toxin has evolved to exploit promiscuous binding properties of ARFs.
 ==About this Structure==
-A5F is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae] with MG, NA, GTP, NAD and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A5F OCA].
+A5F is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=GTP:'>GTP</scene>, <scene name='pdbligand=NAD:'>NAD</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5F OCA].
 ==Reference==
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 [[Category: Protein complex]]
 [[Category: Vibrio cholerae]]
-[[Category: Hol, W.G.J.]]
+[[Category: Hol, W G.J.]]
-[[Category: Holmes, R.K.]]
+[[Category: Holmes, R K.]]
-[[Category: Jobling, M.G.]]
+[[Category: Jobling, M G.]]
-[[Category: Neal, C.J.O.]]
+[[Category: Neal, C J.O.]]
 [[Category: GOL]]
 [[Category: GTP]]
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 [[Category: protein transport/transferase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:46:32 2007''
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