1zse: Difference between revisions

Revision as of 17:18, 21 February 2008

RNA stemloop from bacteriophage Qbeta complexed with an N87S mutant MS2 Capsid

OverviewOverview

Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism.

About this StructureAbout this Structure

1ZSE is a Single protein structure of sequence from Enterobacterio phage ms2. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2., Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG, Structure. 2006 Mar;14(3):487-95. PMID:16531233

Page seeded by OCA on Thu Feb 21 16:18:41 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1zse.gif|left|200px]]<br /><applet load="1zse" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1zse.gif|left|200px]]<br /><applet load="1zse" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1zse, resolution 3.0&Aring;" />
 '''RNA stemloop from bacteriophage Qbeta complexed with an N87S mutant MS2 Capsid'''<br />
 ==Overview==
-Sequence-specific interactions between RNA stem-loops and coat protein, (CP) subunits play vital roles in the life cycles of the RNA, bacteriophages, e.g., by allowing translational repression of their, replicase cistrons and tagging their own RNA genomes for encapsidation., The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of, their cognate translational operators, even in the presence of closely, related operators from other phages in vivo. Discrete mutations within the, MS2 CP have been shown to relax this discrimination in vitro. We have, determined the structures of eight complexes between such mutants and both, MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with, previously determined in vivo repression data, the structures enable us to, propose the molecular basis for the discrimination mechanism.
+Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism.
 ==About this Structure==
-ZSE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZSE OCA].
+ZSE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZSE OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Enterobacterio phage ms2]]
 [[Category: Single protein]]
-[[Category: Adams, C.J.]]
+[[Category: Adams, C J.]]
-[[Category: Baron, A.J.]]
+[[Category: Baron, A J.]]
 [[Category: Grahn, E.]]
 [[Category: Helgstrand, C.]]
-[[Category: Horn, W.T.]]
+[[Category: Horn, W T.]]
 [[Category: Lago, H.]]
 [[Category: Liljas, L.]]
-[[Category: Peabody, D.S.]]
+[[Category: Peabody, D S.]]
-[[Category: Phillips, S.E.V.]]
+[[Category: Phillips, S E.V.]]
-[[Category: Stockley, P.G.]]
+[[Category: Stockley, P G.]]
-[[Category: Stonehouse, N.J.]]
+[[Category: Stonehouse, N J.]]
 [[Category: Tars, K.]]
 [[Category: capsid]]
@@ Line 31: / Line 31: @@
 [[Category: virus/viral protein/rna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:38:13 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:18:41 2008''