1zr2: Difference between revisions
New page: left|200px<br /><applet load="1zr2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zr2, resolution 3.90Å" /> '''Structure of a Synap... |
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[[Image:1zr2.gif|left|200px]]<br /><applet load="1zr2" size=" | [[Image:1zr2.gif|left|200px]]<br /><applet load="1zr2" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1zr2, resolution 3.90Å" /> | caption="1zr2, resolution 3.90Å" /> | ||
'''Structure of a Synaptic gamma-delta Resolvase Tetramer Covalently Linked to two Cleaved DNAs'''<br /> | '''Structure of a Synaptic gamma-delta Resolvase Tetramer Covalently Linked to two Cleaved DNAs'''<br /> | ||
==Overview== | ==Overview== | ||
The structure of a synaptic intermediate of the site-specific recombinase | The structure of a synaptic intermediate of the site-specific recombinase gammadelta resolvase covalently linked through Ser10 to two cleaved duplex DNAs has been determined at 3.4 angstrom resolution. This resolvase, activated for recombination by mutations, forms a tetramer whose structure is substantially changed from that of a presynaptic complex between dimeric resolvase and the cleavage site DNA. Because the two cleaved DNA duplexes that are to be recombined lie on opposite sides of the core tetramer, large movements of both protein and DNA are required to achieve strand exchange. The two dimers linked to the DNAs that are to be recombined are held together by a flat interface. This may allow a 180 degrees rotation of one dimer relative to the other in order to reposition the DNA duplexes for strand exchange. | ||
==About this Structure== | ==About this Structure== | ||
1ZR2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | 1ZR2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZR2 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Grindley, N | [[Category: Grindley, N D.]] | ||
[[Category: Kamtekar, S.]] | [[Category: Kamtekar, S.]] | ||
[[Category: Li, W.]] | [[Category: Li, W.]] | ||
[[Category: Sarkis, G | [[Category: Sarkis, G J.]] | ||
[[Category: Steitz, T | [[Category: Steitz, T A.]] | ||
[[Category: Xiong, Y.]] | [[Category: Xiong, Y.]] | ||
[[Category: flat interface]] | [[Category: flat interface]] | ||
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[[Category: site-specific]] | [[Category: site-specific]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:18:14 2008'' | ||
Revision as of 17:18, 21 February 2008
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Structure of a Synaptic gamma-delta Resolvase Tetramer Covalently Linked to two Cleaved DNAs
OverviewOverview
The structure of a synaptic intermediate of the site-specific recombinase gammadelta resolvase covalently linked through Ser10 to two cleaved duplex DNAs has been determined at 3.4 angstrom resolution. This resolvase, activated for recombination by mutations, forms a tetramer whose structure is substantially changed from that of a presynaptic complex between dimeric resolvase and the cleavage site DNA. Because the two cleaved DNA duplexes that are to be recombined lie on opposite sides of the core tetramer, large movements of both protein and DNA are required to achieve strand exchange. The two dimers linked to the DNAs that are to be recombined are held together by a flat interface. This may allow a 180 degrees rotation of one dimer relative to the other in order to reposition the DNA duplexes for strand exchange.
About this StructureAbout this Structure
1ZR2 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Structure of a synaptic gammadelta resolvase tetramer covalently linked to two cleaved DNAs., Li W, Kamtekar S, Xiong Y, Sarkis GJ, Grindley ND, Steitz TA, Science. 2005 Aug 19;309(5738):1210-5. Epub 2005 Jun 30. PMID:15994378
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