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-[[Image:1zo1.gif|left|200px]]<br /><applet load="1zo1" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1zo1.gif|left|200px]]<br /><applet load="1zo1" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1zo1" />
 '''IF2, IF1, and tRNA fitted to cryo-EM data OF E. COLI 70S initiation complex'''<br />
 ==Overview==
-The 70S ribosome and its complement of factors required for initiation of, translation in E. coli were purified separately and reassembled in vitro, with GDPNP, producing a stable initiation complex (IC) stalled after 70S, assembly. We have obtained a cryo-EM reconstruction of the IC showing, IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP, contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here, adopts a conformation radically different from that seen in the recent, crystal structure of IF2. The C-terminal domain of IF2 binds to the, single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into, a novel orientation at the P site. The GTP binding domain of IF2 binds to, the GTPase-associated center of the 50S subunit in a manner similar to, EF-G and EF-Tu. Additionally, we present evidence for the localization of, IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of, IF2 in the initiation complex.
+The 70S ribosome and its complement of factors required for initiation of translation in E. coli were purified separately and reassembled in vitro with GDPNP, producing a stable initiation complex (IC) stalled after 70S assembly. We have obtained a cryo-EM reconstruction of the IC showing IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here adopts a conformation radically different from that seen in the recent crystal structure of IF2. The C-terminal domain of IF2 binds to the single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into a novel orientation at the P site. The GTP binding domain of IF2 binds to the GTPase-associated center of the 50S subunit in a manner similar to EF-G and EF-Tu. Additionally, we present evidence for the localization of IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of IF2 in the initiation complex.
 ==About this Structure==
-ZO1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZO1 OCA].
+ZO1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZO1 OCA].
 ==Reference==
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 [[Category: Escherichia coli]]
 [[Category: Protein complex]]
-[[Category: Allen, G.S.]]
+[[Category: Allen, G S.]]
 [[Category: Ehrenberg, M.]]
 [[Category: Frank, J.]]
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 [[Category: ribosome]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:34:03 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:17:28 2008''