1znf: Difference between revisions
New page: left|200px<br /> <applet load="1znf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1znf" /> '''THREE-DIMENSIONAL SOLUTION STRUCTURE OF A S... |
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'''THREE-DIMENSIONAL SOLUTION STRUCTURE OF A SINGLE ZINC FINGER DNA-BINDING DOMAIN'''<br /> | '''THREE-DIMENSIONAL SOLUTION STRUCTURE OF A SINGLE ZINC FINGER DNA-BINDING DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
The three-dimensional solution structure of a zinc finger nucleic acid | The three-dimensional solution structure of a zinc finger nucleic acid binding motif has been determined by nuclear magnetic resonance (NMR) spectroscopy. Spectra of a synthetic peptide corresponding to a single zinc finger from the Xenopus protein Xfin yielded distance and dihedral angle constraints that were used to generate structures from distance geometry and restrained molecular dynamics calculations. The zinc finger is an independently folded domain with a compact globular structure in which the zinc atom is bound by two cysteine and two histidine ligands. The polypeptide backbone fold consists of a well-defined helix, starting as alpha and ending as 3(10) helix, packed against two beta strands that are arranged in a hairpin structure. A high density of basic and polar amino acid side chains on the exposed face of the helix are probably involved in DNA binding. | ||
==About this Structure== | ==About this Structure== | ||
1ZNF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with ZN, ACE and NH2 as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1ZNF with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb87_1.html Zinc Fingers]]. Full crystallographic information is available from [http:// | 1ZNF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=ACE:'>ACE</scene> and <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1ZNF with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb87_1.html Zinc Fingers]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZNF OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Xenopus laevis]] | [[Category: Xenopus laevis]] | ||
[[Category: Zinc Fingers]] | [[Category: Zinc Fingers]] | ||
[[Category: Case, D | [[Category: Case, D A.]] | ||
[[Category: Gippert, G | [[Category: Gippert, G P.]] | ||
[[Category: Lee, M | [[Category: Lee, M S.]] | ||
[[Category: Soman, K | [[Category: Soman, K V.]] | ||
[[Category: Wright, P | [[Category: Wright, P E.]] | ||
[[Category: ACE]] | [[Category: ACE]] | ||
[[Category: NH2]] | [[Category: NH2]] | ||
| Line 25: | Line 24: | ||
[[Category: zinc finger dna binding domain]] | [[Category: zinc finger dna binding domain]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:17:17 2008'' | ||
Revision as of 17:17, 21 February 2008
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THREE-DIMENSIONAL SOLUTION STRUCTURE OF A SINGLE ZINC FINGER DNA-BINDING DOMAIN
OverviewOverview
The three-dimensional solution structure of a zinc finger nucleic acid binding motif has been determined by nuclear magnetic resonance (NMR) spectroscopy. Spectra of a synthetic peptide corresponding to a single zinc finger from the Xenopus protein Xfin yielded distance and dihedral angle constraints that were used to generate structures from distance geometry and restrained molecular dynamics calculations. The zinc finger is an independently folded domain with a compact globular structure in which the zinc atom is bound by two cysteine and two histidine ligands. The polypeptide backbone fold consists of a well-defined helix, starting as alpha and ending as 3(10) helix, packed against two beta strands that are arranged in a hairpin structure. A high density of basic and polar amino acid side chains on the exposed face of the helix are probably involved in DNA binding.
About this StructureAbout this Structure
1ZNF is a Single protein structure of sequence from Xenopus laevis with , and as ligands. The following page contains interesting information on the relation of 1ZNF with [Zinc Fingers]. Full crystallographic information is available from OCA.
ReferenceReference
Three-dimensional solution structure of a single zinc finger DNA-binding domain., Lee MS, Gippert GP, Soman KV, Case DA, Wright PE, Science. 1989 Aug 11;245(4918):635-7. PMID:2503871
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