1zjf: Difference between revisions
New page: left|200px<br /><applet load="1zjf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zjf, resolution 2.20Å" /> '''12mer-spd-P4N'''<br ... |
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[[Image:1zjf.gif|left|200px]]<br /><applet load="1zjf" size=" | [[Image:1zjf.gif|left|200px]]<br /><applet load="1zjf" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1zjf, resolution 2.20Å" /> | caption="1zjf, resolution 2.20Å" /> | ||
'''12mer-spd-P4N'''<br /> | '''12mer-spd-P4N'''<br /> | ||
==Overview== | ==Overview== | ||
Three crystal structures containing the entire Sp1 consensus sequence | Three crystal structures containing the entire Sp1 consensus sequence d(GGGGCGGGG) with two or three additional base-pairs on either the 5' or 3' ends and overhangs have been determined. Despite the different lengths of DNA in the pseudo-dodecamers and pseudo-tridecamer, all three structures form A-DNA duplexes that share a common set of crystal contacts, including a T*(G.C) base triplet and a 5'-overhang that flips out and away from the helical axes to form a Hoogsteen base-pair with the 3'-overhang of a symmetry mate. The global conformations of the three structures differ, however, in the widths of their respective major grooves, the lengths of the molecules, and the extent of crystal packing. The structures were determined from crystals grown in an unusual precipitant for A-DNA, polyethylene glycol (PEG) 400, in combination with polyamines or ions; cobalt hexamine for the pseudo-tridecamer, and spermidine for the pseudo-dodecamers. As the Sp1 binding site is a target for antiviral and anticancer drugs, pseudo-dodecamer crystals were soaked with one such antiviral and anticancer compound, P4N. Although P4N was not visualized unambiguously in the electron density maps, the effect of the drug is evident from significant differences in the lattice constants, crystal packing, and overall conformation of the structure. | ||
==About this Structure== | ==About this Structure== | ||
1ZJF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | 1ZJF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZJF OCA]. | ||
==Reference== | ==Reference== | ||
Influence of ions, hydration, and the transcriptional inhibitor P4N on the conformations of the Sp1 binding site., Dohm JA, Hsu MH, Hwu JR, Huang RC, Moudrianakis EN, Lattman EE, Gittis AG, J Mol Biol. 2005 Jun 17;349(4):731-44. Epub 2005 Apr 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15896803 15896803] | Influence of ions, hydration, and the transcriptional inhibitor P4N on the conformations of the Sp1 binding site., Dohm JA, Hsu MH, Hwu JR, Huang RC, Moudrianakis EN, Lattman EE, Gittis AG, J Mol Biol. 2005 Jun 17;349(4):731-44. Epub 2005 Apr 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15896803 15896803] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Dohm, J | [[Category: Dohm, J A.]] | ||
[[Category: Gittis, A | [[Category: Gittis, A G.]] | ||
[[Category: Hsu, M | [[Category: Hsu, M H.]] | ||
[[Category: Huang, R | [[Category: Huang, R C.]] | ||
[[Category: Hwu, J | [[Category: Hwu, J R.]] | ||
[[Category: Lattman, E | [[Category: Lattman, E E.]] | ||
[[Category: Moudrianakis, E | [[Category: Moudrianakis, E N.]] | ||
[[Category: a-dna]] | [[Category: a-dna]] | ||
[[Category: flipped-out bases]] | [[Category: flipped-out bases]] | ||
[[Category: p4n]] | [[Category: p4n]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:16:11 2008'' | ||
Revision as of 17:16, 21 February 2008
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12mer-spd-P4N
OverviewOverview
Three crystal structures containing the entire Sp1 consensus sequence d(GGGGCGGGG) with two or three additional base-pairs on either the 5' or 3' ends and overhangs have been determined. Despite the different lengths of DNA in the pseudo-dodecamers and pseudo-tridecamer, all three structures form A-DNA duplexes that share a common set of crystal contacts, including a T*(G.C) base triplet and a 5'-overhang that flips out and away from the helical axes to form a Hoogsteen base-pair with the 3'-overhang of a symmetry mate. The global conformations of the three structures differ, however, in the widths of their respective major grooves, the lengths of the molecules, and the extent of crystal packing. The structures were determined from crystals grown in an unusual precipitant for A-DNA, polyethylene glycol (PEG) 400, in combination with polyamines or ions; cobalt hexamine for the pseudo-tridecamer, and spermidine for the pseudo-dodecamers. As the Sp1 binding site is a target for antiviral and anticancer drugs, pseudo-dodecamer crystals were soaked with one such antiviral and anticancer compound, P4N. Although P4N was not visualized unambiguously in the electron density maps, the effect of the drug is evident from significant differences in the lattice constants, crystal packing, and overall conformation of the structure.
About this StructureAbout this Structure
1ZJF is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Influence of ions, hydration, and the transcriptional inhibitor P4N on the conformations of the Sp1 binding site., Dohm JA, Hsu MH, Hwu JR, Huang RC, Moudrianakis EN, Lattman EE, Gittis AG, J Mol Biol. 2005 Jun 17;349(4):731-44. Epub 2005 Apr 15. PMID:15896803
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