1zdk: Difference between revisions
New page: left|200px<br /><applet load="1zdk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zdk, resolution 2.86Å" /> '''STRUCTURE OF BACTERI... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1zdk.gif|left|200px]]<br /><applet load="1zdk" size=" | [[Image:1zdk.gif|left|200px]]<br /><applet load="1zdk" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1zdk, resolution 2.86Å" /> | caption="1zdk, resolution 2.86Å" /> | ||
'''STRUCTURE OF BACTERIOPHAGE COAT PROTEIN-LOOP RNA COMPLEX'''<br /> | '''STRUCTURE OF BACTERIOPHAGE COAT PROTEIN-LOOP RNA COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
The coat protein of bacteriophage MS2 is known to bind specifically to an | The coat protein of bacteriophage MS2 is known to bind specifically to an RNA hairpin formed within the MS2 genome. Structurally this hairpin is built up by an RNA double helix interrupted by one unpaired nucleotide and closed by a four-nucleotide loop. We have performed crystallographic studies of complexes between MS2 coat protein capsids and four RNA hairpin variants in order to evaluate the minimal requirements for tight binding to the coat protein and to obtain more information about the three-dimensional structure of these hairpins. An RNA fragment including the four loop nucleotides and a two-base-pair stem but without the unpaired nucleotide is sufficient for binding to the coat protein shell under the conditions used in this study. In contrast, an RNA fragment containing a stem with the unpaired nucleotide but missing the loop nucleotides does not bind to the protein shell. | ||
==About this Structure== | ==About this Structure== | ||
1ZDK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http:// | 1ZDK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZDK OCA]. | ||
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Grahn, E.]] | [[Category: Grahn, E.]] | ||
[[Category: Liljas, L.]] | [[Category: Liljas, L.]] | ||
[[Category: Stonehouse, N | [[Category: Stonehouse, N J.]] | ||
[[Category: Valegard, K.]] | [[Category: Valegard, K.]] | ||
[[Category: Vandenworm, S.]] | [[Category: Vandenworm, S.]] | ||
| Line 25: | Line 25: | ||
[[Category: viral protein capsid]] | [[Category: viral protein capsid]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:14:33 2008'' | ||
Revision as of 17:14, 21 February 2008
|
STRUCTURE OF BACTERIOPHAGE COAT PROTEIN-LOOP RNA COMPLEX
OverviewOverview
The coat protein of bacteriophage MS2 is known to bind specifically to an RNA hairpin formed within the MS2 genome. Structurally this hairpin is built up by an RNA double helix interrupted by one unpaired nucleotide and closed by a four-nucleotide loop. We have performed crystallographic studies of complexes between MS2 coat protein capsids and four RNA hairpin variants in order to evaluate the minimal requirements for tight binding to the coat protein and to obtain more information about the three-dimensional structure of these hairpins. An RNA fragment including the four loop nucleotides and a two-base-pair stem but without the unpaired nucleotide is sufficient for binding to the coat protein shell under the conditions used in this study. In contrast, an RNA fragment containing a stem with the unpaired nucleotide but missing the loop nucleotides does not bind to the protein shell.
About this StructureAbout this Structure
1ZDK is a Single protein structure of sequence from Enterobacterio phage ms2. Full crystallographic information is available from OCA.
ReferenceReference
Crystallographic studies of RNA hairpins in complexes with recombinant MS2 capsids: implications for binding requirements., Grahn E, Stonehouse NJ, Murray JB, van den Worm S, Valegard K, Fridborg K, Stockley PG, Liljas L, RNA. 1999 Jan;5(1):131-8. PMID:9917072
Page seeded by OCA on Thu Feb 21 16:14:33 2008