1z0h: Difference between revisions
New page: left|200px<br /><applet load="1z0h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z0h, resolution 2.00Å" /> '''N-terminal helix reo... |
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[[Image:1z0h.gif|left|200px]]<br /><applet load="1z0h" size=" | [[Image:1z0h.gif|left|200px]]<br /><applet load="1z0h" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1z0h, resolution 2.00Å" /> | caption="1z0h, resolution 2.00Å" /> | ||
'''N-terminal helix reorients in recombinant C-fragment of Clostridium botulinum type B'''<br /> | '''N-terminal helix reorients in recombinant C-fragment of Clostridium botulinum type B'''<br /> | ||
==Overview== | ==Overview== | ||
Botulinum neurotoxins comprise seven distinct serotypes (A-G) produced by | Botulinum neurotoxins comprise seven distinct serotypes (A-G) produced by Clostridium botulinum. The crystal structure of the binding domain of the botulinum neurotoxin type B (BBHc) has been determined to 2A resolution. The overall structure of BBHc is well ordered and similar to that of the binding domain of the holotoxin. However, significant structural changes occur at what would be the interface of translocation and binding domains of the holotoxin. The loop 911-924 shows a maximum displacement of 14.8A at the farthest point. The N-terminal helix reorients and moves by 19.5A from its original position. BBHc is compared with the binding domain of the holotoxin of botulinum type A and B, and the tetanus C-fragment to characterize the heavy chain-carbohydrate interactions. The probable reasons for different binding affinity of botulinum and tetanus toxins are discussed. | ||
==About this Structure== | ==About this Structure== | ||
1Z0H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Active as [http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] Full crystallographic information is available from [http:// | 1Z0H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Active as [http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z0H OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Clostridium botulinum]] | [[Category: Clostridium botulinum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ashraf, S | [[Category: Ashraf, S A.]] | ||
[[Category: Eswarmoorthy, S.]] | [[Category: Eswarmoorthy, S.]] | ||
[[Category: Jayaraman, S.]] | [[Category: Jayaraman, S.]] | ||
[[Category: Smith, L | [[Category: Smith, L A.]] | ||
[[Category: Swaminathan, S.]] | [[Category: Swaminathan, S.]] | ||
[[Category: binding domain]] | [[Category: binding domain]] | ||
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[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:10:57 2008'' | ||
