1yyf: Difference between revisions
New page: left|200px<br /> <applet load="1yyf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yyf, resolution 4.16Å" /> '''Correction of X-ray... |
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[[Image:1yyf.gif|left|200px]]<br /> | [[Image:1yyf.gif|left|200px]]<br /><applet load="1yyf" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1yyf, resolution 4.16Å" /> | caption="1yyf, resolution 4.16Å" /> | ||
'''Correction of X-ray Intensities from an HslV-HslU co-crystal containing lattice translocation defects'''<br /> | '''Correction of X-ray Intensities from an HslV-HslU co-crystal containing lattice translocation defects'''<br /> | ||
==Overview== | ==Overview== | ||
Because of lattice-translocation defects, two identical but translated | Because of lattice-translocation defects, two identical but translated lattices can coexist as a single coherent mosaic block in a crystal. The observed structure in such cases is a weighted sum of two identical but translated structures, one from each lattice; the observed structure factors are a weighted vector sum of the structure factors with identical unit amplitudes but shifted phases. The correction of X-ray intensities from a single crystal containing these defects of the hybrid HslV-HslU complex, which consists of Escherichia coli HslU and Bacillus subtilis HslV (also known as CodW), is reported. When intensities are not corrected, a biologically irrelevant complex (with CodW from one lattice and HslU from another) is implied to exist. Only upon correction does a biologically functional CodW-HslU complex structure emerge. | ||
==About this Structure== | ==About this Structure== | ||
1YYF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1YYF with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http:// | 1YYF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1YYF with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YYF OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Eom, S | [[Category: Eom, S H.]] | ||
[[Category: Park, H | [[Category: Park, H H.]] | ||
[[Category: Rho, S | [[Category: Rho, S H.]] | ||
[[Category: Wang, J.]] | [[Category: Wang, J.]] | ||
[[Category: ADP]] | [[Category: ADP]] | ||
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[[Category: quaternary structure]] | [[Category: quaternary structure]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:10:23 2008'' | ||
Revision as of 17:10, 21 February 2008
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Correction of X-ray Intensities from an HslV-HslU co-crystal containing lattice translocation defects
OverviewOverview
Because of lattice-translocation defects, two identical but translated lattices can coexist as a single coherent mosaic block in a crystal. The observed structure in such cases is a weighted sum of two identical but translated structures, one from each lattice; the observed structure factors are a weighted vector sum of the structure factors with identical unit amplitudes but shifted phases. The correction of X-ray intensities from a single crystal containing these defects of the hybrid HslV-HslU complex, which consists of Escherichia coli HslU and Bacillus subtilis HslV (also known as CodW), is reported. When intensities are not corrected, a biologically irrelevant complex (with CodW from one lattice and HslU from another) is implied to exist. Only upon correction does a biologically functional CodW-HslU complex structure emerge.
About this StructureAbout this Structure
1YYF is a Protein complex structure of sequences from Bacillus subtilis and Escherichia coli with as ligand. The following page contains interesting information on the relation of 1YYF with [AAA+ Proteases]. Full crystallographic information is available from OCA.
ReferenceReference
Correction of X-ray intensities from an HslV-HslU co-crystal containing lattice-translocation defects., Wang J, Rho SH, Park HH, Eom SH, Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):932-41. Epub 2005, Jun 24. PMID:15983416
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