Group:MUZIC:Tritopodin: Difference between revisions

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==Introduction==

Tritopodin is the third member (besides myopodin and synaptopodin) of the podin family ~~proteins~~ and is ~~codified~~ by the gene ''SYNPO2L''[http://www.uniprot.org/uniprot/Q9H987]. Tritopodin has been revealed by database searches, and since it is the third member of the podin family of proteins with extended sequence similarity, was named ‘‘tritopodin’’. ~~Trotopodin~~ is also known under the names synaptopodin 2-like, ~~Myopodin~~-like and CHAP (cytoskeletal heart-enriched actin-associated protein) and is found in heart and skeletal muscle tissue <ref> PMID:19151983 </ref>.

Tritopodin is the third member (besides myopodin and synaptopodin) of the podin protein family and is encoded by the gene ''SYNPO2L''[http://www.uniprot.org/uniprot/Q9H987]. Tritopodin has been revealed by database searches, and since it is the third member of the podin family of proteins with extended sequence similarity, was named ‘‘tritopodin’’. Tritopodin is also known under the names synaptopodin 2-like, myopodin-like and CHAP (cytoskeletal heart-enriched actin-associated protein) and is found in heart and skeletal muscle tissue <ref> PMID:19151983 </ref>.

==Sequence Annotation==

Tritopodin ~~share~~ 33% of homology in the ~~Aminoacid sequence~~ with ~~Myopodin~~ and 30% with Synaptopodin <ref> PMID:9314539 </ref>, which ~~makes believe~~ similar functions for both proteins. The human gene SYNPO2L~~-Gen~~, that ~~codifies for Tritopodin it is form by~~ 5 exons, which are separated by different large introns. Database ~~Analysis~~ (UCSC Genome

Tritopodin shares 33% homology at the aminoacid level with myopodin and 30% with Synaptopodin <ref> PMID:9314539 </ref>, which predicts similar functions for both proteins. The human gene SYNPO2L, that encodes tritopodin exists of 5 exons, which are separated by different large introns. Database analysis (UCSC Genome Browser, EMBL) predicted 2 putative differential isoforms. Splicing isoforms: Tritopodin a (in human 102.5kDa and in mouse 103.3kDa and Tritopodin b (in both human and mouse 79kDa) (see figure). After done biochemicals experiments, Beqqali et al. postulated 2 isoforms: mCHAPa from exon 1,2,3 and 5, as well as mCHAPb that result from a start-codon in exon 4, from which derives two proteins variants of 140kDa (mCHAPa) and 110kDa (mCHAPb) <ref> PMID:20215401 </ref>.

Browser, EMBL) predicted 2 putative differential ~~splicing sequence~~. Splicing sequence 1 lead to of the 4th Exons, while the intraexon splicing sequence 2 lead to the loss of the central portion of the 5th exon. From these splicing sequence and an alternative transcription start were predicted 2 isoforms: Tritopodin a (in human 102.5kDa and in mouse 103.3kDa ~~see figure)~~ and Tritopodin b (in both human and mouse 79kDa). After done biochemicals experiments, Beqqali et al. postulated 2 isoforms: mCHAPa from exon 1,2,3 and 5, as well as mCHAPb that result from a start-codon in exon 4, from which derives two proteins variants of 140kDa (mCHAPa) and 110kDa (mCHAPb) <ref> PMID:20215401 </ref>.

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==Function==

Tritopodin is expressed in vivo and in vitro in differentiated skeletal- and heart muscle cells~~, associates with polymerized actin~~, and is conserved amongst vertebrates, indicating an essential role in muscle function. Furthermore Tritopodin is able to translocate to the nucleus, and plays an important role in skeletal and cardiac muscle development. <ref> PMID:20554076 </ref> <ref> PMID:20215401 </ref>.

Tritopodin is expressed in vivo and in vitro in differentiated skeletal- and heart muscle cells, and is conserved amongst vertebrates, indicating an essential role in muscle function. Furthermore Tritopodin is able to translocate to the nucleus, and plays an important role in skeletal and cardiac muscle development. <ref> PMID:20554076 </ref> <ref> PMID:20215401 </ref>.

==Tritopodin Interactions==

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 ==Introduction==
-Tritopodin is the third member (besides myopodin and synaptopodin) of the podin family proteins and is codified by the gene ''SYNPO2L''[http://www.uniprot.org/uniprot/Q9H987]. Tritopodin has been revealed by database searches, and since it is the third member  of the podin family of proteins with extended sequence similarity, was named ‘‘tritopodin’’. Trotopodin is also known under the names synaptopodin 2-like, Myopodin-like and CHAP (cytoskeletal heart-enriched actin-associated protein) and is found in heart and skeletal muscle tissue <ref> PMID:19151983 </ref>.
+Tritopodin is the third member (besides myopodin and synaptopodin) of the podin protein family and is encoded by the gene ''SYNPO2L''[http://www.uniprot.org/uniprot/Q9H987]. Tritopodin has been revealed by database searches, and since it is the third member  of the podin family of proteins with extended sequence similarity, was named ‘‘tritopodin’’. Tritopodin is also known under the names synaptopodin 2-like, myopodin-like and CHAP (cytoskeletal heart-enriched actin-associated protein) and is found in heart and skeletal muscle tissue <ref> PMID:19151983 </ref>.
 ==Sequence Annotation==
-Tritopodin share 33% of homology in the Aminoacid sequence with Myopodin and 30% with Synaptopodin <ref> PMID:9314539 </ref>, which makes believe similar functions for both proteins. The human gene SYNPO2L-Gen, that codifies for Tritopodin it is form by 5 exons, which are separated by different large introns. Database Analysis (UCSC Genome
+Tritopodin shares 33% homology at the aminoacid level with myopodin and 30% with Synaptopodin <ref> PMID:9314539 </ref>, which predicts similar functions for both proteins. The human gene SYNPO2L, that encodes tritopodin exists of 5 exons, which are separated by different large introns. Database analysis (UCSC Genome Browser, EMBL) predicted 2 putative differential isoforms. Splicing isoforms: Tritopodin a (in human 102.5kDa and in mouse 103.3kDa and Tritopodin b (in both human and mouse 79kDa) (see figure). After done biochemicals experiments, Beqqali et al. postulated 2 isoforms: mCHAPa from exon 1,2,3 and 5, as well as mCHAPb that result from a start-codon in exon 4, from which derives two proteins variants of 140kDa (mCHAPa) and 110kDa (mCHAPb) <ref> PMID:20215401 </ref>.
-Browser, EMBL) predicted 2 putative differential splicing sequence. Splicing sequence 1 lead to of the 4th Exons, while the intraexon splicing sequence 2 lead to the loss of the central portion of the 5th exon. From these splicing sequence and an alternative transcription start were predicted 2 isoforms: Tritopodin a (in human 102.5kDa and in mouse 103.3kDa see figure) and Tritopodin b (in both human and mouse 79kDa). After done biochemicals experiments, Beqqali et al. postulated 2 isoforms: mCHAPa from exon 1,2,3 and 5, as well as mCHAPb that result from a start-codon in exon 4, from which derives two proteins variants of 140kDa (mCHAPa) and 110kDa (mCHAPb) <ref> PMID:20215401 </ref>.
@@ Line 13: / Line 12: @@
 ==Function==
-Tritopodin is expressed in vivo and in vitro in differentiated skeletal- and heart muscle cells, associates with polymerized actin, and is conserved amongst vertebrates, indicating an essential role in muscle function. Furthermore Tritopodin is able to translocate to the nucleus, and plays an important role in skeletal and cardiac muscle development. <ref> PMID:20554076 </ref> <ref> PMID:20215401 </ref>.
+Tritopodin is expressed in vivo and in vitro in differentiated skeletal- and heart muscle cells, and is conserved amongst vertebrates, indicating an essential role in muscle function. Furthermore Tritopodin is able to translocate to the nucleus, and plays an important role in skeletal and cardiac muscle development. <ref> PMID:20554076 </ref> <ref> PMID:20215401 </ref>.
 ==Tritopodin Interactions==