Group:MUZIC:Tritopodin: Difference between revisions

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==Sequence Annotation==

Tritopodin share 33% of homology in the Aminoacid sequence with Myopodin and 30% with Synaptopodin <ref> PMID:9314539 </ref>, which makes believe similar functions for both proteins. The human gene SYNPO2L-Gen, that codifies for Tritopodin it is form by 5 exons, which are separated by different large introns. Database Analysis (UCSC Genome

Browser, EMBL) predicted 2 putative differential splicing sequence. Splicing sequence 1 lead to of the 4th Exons, while the intraexon splicing sequence 2 lead to the loss of the central portion of the 5th exon. From these splicing sequence and an alternative transcription start were predicted 2 isoforms: Tritopodin a (in human 102.5kDa and in mouse 103.3kDa see figure) and Tritopodin b (in both human and mouse 79kDa). After ~~running SDS gel~~, Beqqali et al. postulated 2 isoforms: mCHAPa from exon 1,2,3 and 5, as well as mCHAPb that result from a start-codon in exon 4, from which derives two proteins variants of 140kDa (mCHAPa) and 110kDa (mCHAPb) <ref> PMID:20215401 </ref>.

Browser, EMBL) predicted 2 putative differential splicing sequence. Splicing sequence 1 lead to of the 4th Exons, while the intraexon splicing sequence 2 lead to the loss of the central portion of the 5th exon. From these splicing sequence and an alternative transcription start were predicted 2 isoforms: Tritopodin a (in human 102.5kDa and in mouse 103.3kDa see figure) and Tritopodin b (in both human and mouse 79kDa). After done biochemicals experiments, Beqqali et al. postulated 2 isoforms: mCHAPa from exon 1,2,3 and 5, as well as mCHAPb that result from a start-codon in exon 4, from which derives two proteins variants of 140kDa (mCHAPa) and 110kDa (mCHAPb) <ref> PMID:20215401 </ref>.

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 ==Sequence Annotation==
 Tritopodin share 33% of homology in the Aminoacid sequence with Myopodin and 30% with Synaptopodin <ref> PMID:9314539 </ref>, which makes believe similar functions for both proteins. The human gene SYNPO2L-Gen, that codifies for Tritopodin it is form by 5 exons, which are separated by different large introns. Database Analysis (UCSC Genome
-Browser, EMBL) predicted 2 putative differential splicing sequence. Splicing sequence 1 lead to of the 4th Exons, while the intraexon splicing sequence 2 lead to the loss of the central portion of the 5th exon. From these splicing sequence and an alternative transcription start were predicted 2 isoforms: Tritopodin a (in human 102.5kDa and in mouse 103.3kDa see figure) and Tritopodin b (in both human and mouse 79kDa). After running SDS gel, Beqqali et al. postulated 2 isoforms: mCHAPa from exon 1,2,3 and 5, as well as mCHAPb that result from a start-codon in exon 4, from which derives two proteins variants of 140kDa (mCHAPa) and 110kDa (mCHAPb) <ref> PMID:20215401 </ref>.
+Browser, EMBL) predicted 2 putative differential splicing sequence. Splicing sequence 1 lead to of the 4th Exons, while the intraexon splicing sequence 2 lead to the loss of the central portion of the 5th exon. From these splicing sequence and an alternative transcription start were predicted 2 isoforms: Tritopodin a (in human 102.5kDa and in mouse 103.3kDa see figure) and Tritopodin b (in both human and mouse 79kDa). After done biochemicals experiments, Beqqali et al. postulated 2 isoforms: mCHAPa from exon 1,2,3 and 5, as well as mCHAPb that result from a start-codon in exon 4, from which derives two proteins variants of 140kDa (mCHAPa) and 110kDa (mCHAPb) <ref> PMID:20215401 </ref>.