1y1j: Difference between revisions
New page: left|200px<br /> <applet load="1y1j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1y1j, resolution 1.55Å" /> '''human formylglycine... |
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[[Image:1y1j.gif|left|200px]]<br /> | [[Image:1y1j.gif|left|200px]]<br /><applet load="1y1j" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1y1j, resolution 1.55Å" /> | caption="1y1j, resolution 1.55Å" /> | ||
'''human formylglycine generating enzyme, sulfonic acid/desulfurated form'''<br /> | '''human formylglycine generating enzyme, sulfonic acid/desulfurated form'''<br /> | ||
==Overview== | ==Overview== | ||
Sulfatases are enzymes essential for degradation and remodeling of sulfate | Sulfatases are enzymes essential for degradation and remodeling of sulfate esters. Formylglycine (FGly), the key catalytic residue in the active site, is unique to sulfatases. In higher eukaryotes, FGly is generated from a cysteine precursor by the FGly-generating enzyme (FGE). Inactivity of FGE results in multiple sulfatase deficiency (MSD), a fatal autosomal recessive syndrome. Based on the crystal structure, we report that FGE is a single-domain monomer with a surprising paucity of secondary structure and adopts a unique fold. The effect of all 18 missense mutations found in MSD patients is explained by the FGE structure, providing a molecular basis of MSD. The catalytic mechanism of FGly generation was elucidated by six high-resolution structures of FGE in different redox environments. The structures allow formulation of a novel oxygenase mechanism whereby FGE utilizes molecular oxygen to generate FGly via a cysteine sulfenic acid intermediate. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1Y1J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA and CXS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1Y1J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=CXS:'>CXS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y1J OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Dickmanns, A.]] | [[Category: Dickmanns, A.]] | ||
[[Category: Ficner, R.]] | [[Category: Ficner, R.]] | ||
[[Category: Rudolph, M | [[Category: Rudolph, M G.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
[[Category: CXS]] | [[Category: CXS]] | ||
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[[Category: multiple sulfatase deficiency]] | [[Category: multiple sulfatase deficiency]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:00:53 2008'' | ||
Revision as of 17:00, 21 February 2008
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human formylglycine generating enzyme, sulfonic acid/desulfurated form
OverviewOverview
Sulfatases are enzymes essential for degradation and remodeling of sulfate esters. Formylglycine (FGly), the key catalytic residue in the active site, is unique to sulfatases. In higher eukaryotes, FGly is generated from a cysteine precursor by the FGly-generating enzyme (FGE). Inactivity of FGE results in multiple sulfatase deficiency (MSD), a fatal autosomal recessive syndrome. Based on the crystal structure, we report that FGE is a single-domain monomer with a surprising paucity of secondary structure and adopts a unique fold. The effect of all 18 missense mutations found in MSD patients is explained by the FGE structure, providing a molecular basis of MSD. The catalytic mechanism of FGly generation was elucidated by six high-resolution structures of FGE in different redox environments. The structures allow formulation of a novel oxygenase mechanism whereby FGE utilizes molecular oxygen to generate FGly via a cysteine sulfenic acid intermediate.
DiseaseDisease
Known disease associated with this structure: Multiple sulfatase deficiency OMIM:[607939]
About this StructureAbout this Structure
1Y1J is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Molecular basis for multiple sulfatase deficiency and mechanism for formylglycine generation of the human formylglycine-generating enzyme., Dierks T, Dickmanns A, Preusser-Kunze A, Schmidt B, Mariappan M, von Figura K, Ficner R, Rudolph MG, Cell. 2005 May 20;121(4):541-52. PMID:15907468
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