1xqh: Difference between revisions
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==Overview== | ==Overview== | ||
p53 is a tumour suppressor that regulates the cellular response to | p53 is a tumour suppressor that regulates the cellular response to genotoxic stresses. p53 is a short-lived protein and its activity is regulated mostly by stabilization via different post-translational modifications. Here we report a novel mechanism of p53 regulation through lysine methylation by Set9 methyltransferase. Set9 specifically methylates p53 at one residue within the carboxyl-terminus regulatory region. Methylated p53 is restricted to the nucleus and the modification positively affects its stability. Set9 regulates the expression of p53 target genes in a manner dependent on the p53-methylation site. The crystal structure of a ternary complex of Set9 with a p53 peptide and the cofactor product S-adenosyl-l-homocysteine (AdoHcy) provides the molecular basis for recognition of p53 by this lysine methyltransferase. | ||
==Disease== | ==Disease== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Barlev, N | [[Category: Barlev, N A.]] | ||
[[Category: Chuikov, S.]] | [[Category: Chuikov, S.]] | ||
[[Category: Gamblin, S | [[Category: Gamblin, S J.]] | ||
[[Category: Ivanov, G | [[Category: Ivanov, G S.]] | ||
[[Category: Justin, N.]] | [[Category: Justin, N.]] | ||
[[Category: Kurash, J | [[Category: Kurash, J K.]] | ||
[[Category: McKinney, K.]] | [[Category: McKinney, K.]] | ||
[[Category: Prives, C.]] | [[Category: Prives, C.]] | ||
[[Category: Reinberg, D.]] | [[Category: Reinberg, D.]] | ||
[[Category: Tempst, P.]] | [[Category: Tempst, P.]] | ||
[[Category: Wilson, J | [[Category: Wilson, J R.]] | ||
[[Category: Xiao, B.]] | [[Category: Xiao, B.]] | ||
[[Category: SAH]] | [[Category: SAH]] | ||
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[[Category: set9-p53 complex]] | [[Category: set9-p53 complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:57:35 2008'' | ||
Revision as of 16:57, 21 February 2008
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Crystal structure of a ternary complex of the methyltransferase SET9 (also known as SET7/9) with a P53 peptide and SAH
OverviewOverview
p53 is a tumour suppressor that regulates the cellular response to genotoxic stresses. p53 is a short-lived protein and its activity is regulated mostly by stabilization via different post-translational modifications. Here we report a novel mechanism of p53 regulation through lysine methylation by Set9 methyltransferase. Set9 specifically methylates p53 at one residue within the carboxyl-terminus regulatory region. Methylated p53 is restricted to the nucleus and the modification positively affects its stability. Set9 regulates the expression of p53 target genes in a manner dependent on the p53-methylation site. The crystal structure of a ternary complex of Set9 with a p53 peptide and the cofactor product S-adenosyl-l-homocysteine (AdoHcy) provides the molecular basis for recognition of p53 by this lysine methyltransferase.
DiseaseDisease
Known diseases associated with this structure: Adrenal cortical carcinoma OMIM:[191170], Breast cancer OMIM:[191170], Colorectal cancer OMIM:[191170], Hepatocellular carcinoma OMIM:[191170], Histiocytoma OMIM:[191170], Li-Fraumeni syndrome OMIM:[191170], Multiple malignancy syndrome OMIM:[191170], Nasopharyngeal carcinoma OMIM:[191170], Osteosarcoma OMIM:[191170], Pancreatic cancer OMIM:[191170], Thyroid carcinoma OMIM:[191170]
About this StructureAbout this Structure
1XQH is a Protein complex structure of sequences from Homo sapiens with as ligand. Active as Histone-lysine N-methyltransferase, with EC number 2.1.1.43 Full crystallographic information is available from OCA.
ReferenceReference
Regulation of p53 activity through lysine methylation., Chuikov S, Kurash JK, Wilson JR, Xiao B, Justin N, Ivanov GS, McKinney K, Tempst P, Prives C, Gamblin SJ, Barlev NA, Reinberg D, Nature. 2004 Nov 18;432(7015):353-60. Epub 2004 Nov 3. PMID:15525938
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