1xib: Difference between revisions

Revision as of 16:55, 21 February 2008

MODES OF BINDING SUBSTRATES AND THEIR ANALOGUES TO THE ENZYME D-XYLOSE ISOMERASE

OverviewOverview

Studies of binding of substrates and inhibitors of the enzyme D-xylose isomerase show, from X-ray diffraction data at 1.6-1.9 A resolution, that there are a variety of binding modes. These vary in the manner in which the substrate or its analogue extend, on binding, across the carboxy end of the (betaalpha)(8)-barrel structure. These binding sites are His54 and the metal ion (magnesium or manganese) that is held in place by Glul81, Asp245, Glu217 and Asp287. Possible catalytic groups have been identified in proposed mechanisms and their role in the binding of ligands is illustrated.

About this StructureAbout this Structure

1XIB is a Single protein structure of sequence from Streptomyces rubiginosus with as ligand. This structure supersedes the now removed PDB entries 7XIA and 2XIA. Active as Xylose isomerase, with EC number 5.3.1.5 Full crystallographic information is available from OCA.

ReferenceReference

Modes of binding substrates and their analogues to the enzyme D-xylose isomerase., Carrell HL, Hoier H, Glusker JP, Acta Crystallogr D Biol Crystallogr. 1994 Mar 1;50(Pt 2):113-23. PMID:15299449

Page seeded by OCA on Thu Feb 21 15:55:12 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1xib.jpg|left|200px]]<br /><applet load="1xib" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1xib.jpg|left|200px]]<br /><applet load="1xib" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1xib, resolution 1.6&Aring;" />
 '''MODES OF BINDING SUBSTRATES AND THEIR ANALOGUES TO THE ENZYME D-XYLOSE ISOMERASE'''<br />
 ==Overview==
-Studies of binding of substrates and inhibitors of the enzyme D-xylose, isomerase show, from X-ray diffraction data at 1.6-1.9 A resolution, that, there are a variety of binding modes. These vary in the manner in which, the substrate or its analogue extend, on binding, across the carboxy end, of the (betaalpha)(8)-barrel structure. These binding sites are His54 and, the metal ion (magnesium or manganese) that is held in place by Glul81, Asp245, Glu217 and Asp287. Possible catalytic groups have been identified, in proposed mechanisms and their role in the binding of ligands is, illustrated.
+Studies of binding of substrates and inhibitors of the enzyme D-xylose isomerase show, from X-ray diffraction data at 1.6-1.9 A resolution, that there are a variety of binding modes. These vary in the manner in which the substrate or its analogue extend, on binding, across the carboxy end of the (betaalpha)(8)-barrel structure. These binding sites are His54 and the metal ion (magnesium or manganese) that is held in place by Glul81, Asp245, Glu217 and Asp287. Possible catalytic groups have been identified in proposed mechanisms and their role in the binding of ligands is illustrated.
 ==About this Structure==
-XIB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. This structure superseeds the now removed PDB entries 7XIA and 2XIA. Active as [http://en.wikipedia.org/wiki/Xylose_isomerase Xylose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.5 5.3.1.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XIB OCA].
+XIB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure supersedes the now removed PDB entries 7XIA and 2XIA. Active as [http://en.wikipedia.org/wiki/Xylose_isomerase Xylose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.5 5.3.1.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XIB OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Streptomyces rubiginosus]]
 [[Category: Xylose isomerase]]
-[[Category: Carrell, H.L.]]
+[[Category: Carrell, H L.]]
-[[Category: Glusker, J.P.]]
+[[Category: Glusker, J P.]]
 [[Category: MN]]
 [[Category: isomerase(intramolecular oxidoreductse)]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:05:19 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:55:12 2008''