1xfe: Difference between revisions
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'''Solution structure of the LA7-EGFA pair from the LDL receptor'''<br /> | '''Solution structure of the LA7-EGFA pair from the LDL receptor'''<br /> | ||
==Overview== | ==Overview== | ||
Low-density lipoprotein (LDL) receptors bind lipoprotein particles at the | Low-density lipoprotein (LDL) receptors bind lipoprotein particles at the cell surface and release them in the low pH environment of the endosome. The published structure of the receptor determined at endosomal pH reveals an interdomain interface between its beta propeller and its fourth and fifth ligand binding (LA) repeats, suggesting that the receptor adopts a closed conformation at low pH to release LDL. Here, we combine lipoprotein binding and release assays with NMR spectroscopy to examine structural features of the receptor promoting release of LDL at low pH. These studies lead to a model in which the receptor uses a pH-invariant scaffold as an anchor to restrict conformational search space, combining it with flexible linkers between ligand binding repeats to interconvert between open and closed conformations. This finely tuned balance between interdomain rigidity and flexibility is likely to represent a shared structural feature in proteins of the LDL receptor family. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1XFE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1XFE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XFE OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Beglova, N.]] | [[Category: Beglova, N.]] | ||
[[Category: Blacklow, S | [[Category: Blacklow, S C.]] | ||
[[Category: Fisher, C.]] | [[Category: Fisher, C.]] | ||
[[Category: Jeon, H.]] | [[Category: Jeon, H.]] | ||
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[[Category: ligand-binding repeat]] | [[Category: ligand-binding repeat]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:54:17 2008'' | ||
Revision as of 16:54, 21 February 2008
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Solution structure of the LA7-EGFA pair from the LDL receptor
OverviewOverview
Low-density lipoprotein (LDL) receptors bind lipoprotein particles at the cell surface and release them in the low pH environment of the endosome. The published structure of the receptor determined at endosomal pH reveals an interdomain interface between its beta propeller and its fourth and fifth ligand binding (LA) repeats, suggesting that the receptor adopts a closed conformation at low pH to release LDL. Here, we combine lipoprotein binding and release assays with NMR spectroscopy to examine structural features of the receptor promoting release of LDL at low pH. These studies lead to a model in which the receptor uses a pH-invariant scaffold as an anchor to restrict conformational search space, combining it with flexible linkers between ligand binding repeats to interconvert between open and closed conformations. This finely tuned balance between interdomain rigidity and flexibility is likely to represent a shared structural feature in proteins of the LDL receptor family.
DiseaseDisease
Known disease associated with this structure: Hypercholesterolemia, familial OMIM:[606945]
About this StructureAbout this Structure
1XFE is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Cooperation between fixed and low pH-inducible interfaces controls lipoprotein release by the LDL receptor., Beglova N, Jeon H, Fisher C, Blacklow SC, Mol Cell. 2004 Oct 22;16(2):281-92. PMID:15494314
Page seeded by OCA on Thu Feb 21 15:54:17 2008