1xed: Difference between revisions
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==Overview== | ==Overview== | ||
The polymeric immunoglobulin receptor (pIgR) is a type I transmembrane | The polymeric immunoglobulin receptor (pIgR) is a type I transmembrane protein that delivers dimeric IgA (dIgA) and pentameric IgM to mucosal secretions. Here, we report the 1.9 A resolution X-ray crystal structure of the N-terminal domain of human pIgR, which binds dIgA in the absence of other pIgR domains with an equilibrium dissociation constant of 300 nM. The structure of pIgR domain 1 reveals a folding topology similar to immunoglobulin variable domains, but with differences in the counterparts of the complementarity determining regions (CDRs), including a helical turn in CDR1 and a CDR3 loop that points away from the other CDRs. The unusual CDR3 loop position prevents dimerization analogous to the pairing of antibody variable heavy and variable light domains. The pIgR domain 1 structure allows interpretation of previous mutagenesis results and structure-based comparisons between pIgR and other IgA receptors. | ||
==Disease== | ==Disease== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bjorkman, P | [[Category: Bjorkman, P J.]] | ||
[[Category: Hamburger, A | [[Category: Hamburger, A E.]] | ||
[[Category: Jr., A | [[Category: Jr., A P.West.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: ig-like fold]] | [[Category: ig-like fold]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:53:53 2008'' | ||
Revision as of 16:53, 21 February 2008
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Crystal Structure of a Ligand-Binding Domain of the Human Polymeric Ig Receptor, pIgR
OverviewOverview
The polymeric immunoglobulin receptor (pIgR) is a type I transmembrane protein that delivers dimeric IgA (dIgA) and pentameric IgM to mucosal secretions. Here, we report the 1.9 A resolution X-ray crystal structure of the N-terminal domain of human pIgR, which binds dIgA in the absence of other pIgR domains with an equilibrium dissociation constant of 300 nM. The structure of pIgR domain 1 reveals a folding topology similar to immunoglobulin variable domains, but with differences in the counterparts of the complementarity determining regions (CDRs), including a helical turn in CDR1 and a CDR3 loop that points away from the other CDRs. The unusual CDR3 loop position prevents dimerization analogous to the pairing of antibody variable heavy and variable light domains. The pIgR domain 1 structure allows interpretation of previous mutagenesis results and structure-based comparisons between pIgR and other IgA receptors.
DiseaseDisease
Known disease associated with this structure: IgA nephropathy, susceptibility to OMIM:[173880]
About this StructureAbout this Structure
1XED is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a polymeric immunoglobulin binding fragment of the human polymeric immunoglobulin receptor., Hamburger AE, West AP Jr, Bjorkman PJ, Structure. 2004 Nov;12(11):1925-35. PMID:15530357
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