1x8s: Difference between revisions

Revision as of 16:52, 21 February 2008

Structure of the Par-6 PDZ domain with a Pals1 internal ligand

OverviewOverview

PDZ protein interaction domains are typically selective for C-terminal ligands, but non-C-terminal, 'internal' ligands have also been identified. The PDZ domain from the cell polarity protein Par-6 binds C-terminal ligands and an internal sequence from the protein Pals1/Stardust. The structure of the Pals1-Par-6 PDZ complex reveals that the PDZ ligand-binding site is deformed to allow for internal binding. Whereas binding of the Rho GTPase Cdc42 to a CRIB domain adjacent to the Par-6 PDZ regulates binding of C-terminal ligands, the conformational change that occurs upon binding of Pals1 renders its binding independent of Cdc42. These results suggest a mechanism by which the requirement for a C terminus can be readily bypassed by PDZ ligands and reveal a complex set of cooperative and competitive interactions in Par-6 that are likely to be important for cell polarity regulation.

About this StructureAbout this Structure

1X8S is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

ReferenceReference

Internal recognition through PDZ domain plasticity in the Par-6-Pals1 complex., Penkert RR, DiVittorio HM, Prehoda KE, Nat Struct Mol Biol. 2004 Nov;11(11):1122-7. Epub 2004 Oct 10. PMID:15475968

Page seeded by OCA on Thu Feb 21 15:52:14 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1x8s.jpg|left|200px]]<br /><applet load="1x8s" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1x8s.jpg|left|200px]]<br /><applet load="1x8s" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1x8s, resolution 2.503&Aring;" />
 '''Structure of the Par-6 PDZ domain with a Pals1 internal ligand'''<br />
 ==Overview==
-PDZ protein interaction domains are typically selective for C-terminal, ligands, but non-C-terminal, 'internal' ligands have also been identified., The PDZ domain from the cell polarity protein Par-6 binds C-terminal, ligands and an internal sequence from the protein Pals1/Stardust. The, structure of the Pals1-Par-6 PDZ complex reveals that the PDZ, ligand-binding site is deformed to allow for internal binding. Whereas, binding of the Rho GTPase Cdc42 to a CRIB domain adjacent to the Par-6 PDZ, regulates binding of C-terminal ligands, the conformational change that, occurs upon binding of Pals1 renders its binding independent of Cdc42., These results suggest a mechanism by which the requirement for a C, terminus can be readily bypassed by PDZ ligands and reveal a complex set, of cooperative and competitive interactions in Par-6 that are likely to be, important for cell polarity regulation.
+PDZ protein interaction domains are typically selective for C-terminal ligands, but non-C-terminal, 'internal' ligands have also been identified. The PDZ domain from the cell polarity protein Par-6 binds C-terminal ligands and an internal sequence from the protein Pals1/Stardust. The structure of the Pals1-Par-6 PDZ complex reveals that the PDZ ligand-binding site is deformed to allow for internal binding. Whereas binding of the Rho GTPase Cdc42 to a CRIB domain adjacent to the Par-6 PDZ regulates binding of C-terminal ligands, the conformational change that occurs upon binding of Pals1 renders its binding independent of Cdc42. These results suggest a mechanism by which the requirement for a C terminus can be readily bypassed by PDZ ligands and reveal a complex set of cooperative and competitive interactions in Par-6 that are likely to be important for cell polarity regulation.
 ==About this Structure==
-X8S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1X8S OCA].
+X8S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8S OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Drosophila melanogaster]]
 [[Category: Single protein]]
-[[Category: DiVittorio, H.M.]]
+[[Category: DiVittorio, H M.]]
-[[Category: Penkert, R.R.]]
+[[Category: Penkert, R R.]]
-[[Category: Prehoda, K.E.]]
+[[Category: Prehoda, K E.]]
 [[Category: cell cycle]]
 [[Category: par-6]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:38:54 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:52:14 2008''