1wxg: Difference between revisions
New page: left|200px<br /><applet load="1wxg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wxg, resolution 1.9Å" /> '''E.coli NAD Synthetase... |
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[[Image:1wxg.gif|left|200px]]<br /><applet load="1wxg" size=" | [[Image:1wxg.gif|left|200px]]<br /><applet load="1wxg" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1wxg, resolution 1.9Å" /> | caption="1wxg, resolution 1.9Å" /> | ||
'''E.coli NAD Synthetase, DND'''<br /> | '''E.coli NAD Synthetase, DND'''<br /> | ||
==Overview== | ==Overview== | ||
Nicotinamide adenine dinucleotide synthetases (NADS) catalyze the | Nicotinamide adenine dinucleotide synthetases (NADS) catalyze the amidation of nicotinic acid adenine dinucleotide (NAAD) to yield the enzyme cofactor nicotinamide adenine dinucleotide (NAD). Here we describe the crystal structures of the ammonia-dependent homodimeric NADS from Escherichia coli alone and in complex with natural substrates and with the reaction product NAD. The structures disclosed two NAAD/NAD binding sites at the dimer interface and an adenosine triphosphate (ATP) binding site within each subunit. Comparison with the Bacillus subtilis NADS showed pronounced chemical differences in the NAAD/NAD binding sites and less prominent differences in the ATP binding pockets. In addition, the E. coli NADS structures revealed unexpected dynamical rearrangements in the NAAD/NAD binding pocket upon NAAD-to-NAD conversion, which define a catalysis state and a substrate/product exchange state. The two states are adopted by concerted movement of the nicotinysyl moieties of NAAD and NAD, Phe-170, and residues 224-228, which may be triggered by differential coordination of a magnesium ion to NAAD and NAD. Phylogenetic structure comparisons suggest that the present results are relevant for designing species-specific antibiotics. | ||
==About this Structure== | ==About this Structure== | ||
1WXG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and DND as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/NAD(+)_synthase NAD(+) synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.5 6.3.1.5] Full crystallographic information is available from [http:// | 1WXG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=DND:'>DND</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/NAD(+)_synthase NAD(+) synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.5 6.3.1.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WXG OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Humm, A.]] | [[Category: Humm, A.]] | ||
[[Category: Jauch, R.]] | [[Category: Jauch, R.]] | ||
[[Category: Wahl, M | [[Category: Wahl, M C.]] | ||
[[Category: DND]] | [[Category: DND]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: e | [[Category: e coli]] | ||
[[Category: nad]] | [[Category: nad]] | ||
[[Category: nade]] | [[Category: nade]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:49:07 2008'' | ||
Revision as of 16:49, 21 February 2008
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E.coli NAD Synthetase, DND
OverviewOverview
Nicotinamide adenine dinucleotide synthetases (NADS) catalyze the amidation of nicotinic acid adenine dinucleotide (NAAD) to yield the enzyme cofactor nicotinamide adenine dinucleotide (NAD). Here we describe the crystal structures of the ammonia-dependent homodimeric NADS from Escherichia coli alone and in complex with natural substrates and with the reaction product NAD. The structures disclosed two NAAD/NAD binding sites at the dimer interface and an adenosine triphosphate (ATP) binding site within each subunit. Comparison with the Bacillus subtilis NADS showed pronounced chemical differences in the NAAD/NAD binding sites and less prominent differences in the ATP binding pockets. In addition, the E. coli NADS structures revealed unexpected dynamical rearrangements in the NAAD/NAD binding pocket upon NAAD-to-NAD conversion, which define a catalysis state and a substrate/product exchange state. The two states are adopted by concerted movement of the nicotinysyl moieties of NAAD and NAD, Phe-170, and residues 224-228, which may be triggered by differential coordination of a magnesium ion to NAAD and NAD. Phylogenetic structure comparisons suggest that the present results are relevant for designing species-specific antibiotics.
About this StructureAbout this Structure
1WXG is a Single protein structure of sequence from Escherichia coli with and as ligands. Active as NAD(+) synthase, with EC number 6.3.1.5 Full crystallographic information is available from OCA.
ReferenceReference
Structures of Escherichia coli NAD synthetase with substrates and products reveal mechanistic rearrangements., Jauch R, Humm A, Huber R, Wahl MC, J Biol Chem. 2005 Apr 15;280(15):15131-40. Epub 2005 Feb 7. PMID:15699042
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