1wlh: Difference between revisions

Revision as of 16:45, 21 February 2008

Molecular structure of the rod domain of Dictyostelium filamin

OverviewOverview

Dictyostelium discoideum filamin (ddFLN) is a two-chain F-actin crosslinking protein with an N-terminal actin-binding domain and a rod domain constructed from six tandem repeats of a 100 residue motif that has an immunoglobulin (Ig) fold. We report the 2.8 A resolution crystal structure of a homodimer of rod repeats 4, 5 and 6. The two chains are arranged in an antiparallel fashion and form an elongated element, which is shortened, however, compared to a fully extended, linear configuration because the long axis of each Ig domain is arranged at an angle to the long axis of the rod. Same arrangement of repeats should also be present in the rod domain of human FLNa, much longer than Dictyostelium FLN, which forms an extended structure able to crosslink F-actin chains over distances of more than 1000 A.

About this StructureAbout this Structure

1WLH is a Single protein structure of sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA.

ReferenceReference

Molecular structure of the rod domain of dictyostelium filamin., Popowicz GM, Muller R, Noegel AA, Schleicher M, Huber R, Holak TA, J Mol Biol. 2004 Oct 1;342(5):1637-46. PMID:15364587

Page seeded by OCA on Thu Feb 21 15:45:37 2008

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OCA

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-[[Image:1wlh.jpg|left|200px]]<br /><applet load="1wlh" size="450" color="white" frame="true" align="right" spinBox="true"
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 caption="1wlh, resolution 2.80&Aring;" />
 '''Molecular structure of the rod domain of Dictyostelium filamin'''<br />
 ==Overview==
-Dictyostelium discoideum filamin (ddFLN) is a two-chain F-actin, crosslinking protein with an N-terminal actin-binding domain and a rod, domain constructed from six tandem repeats of a 100 residue motif that has, an immunoglobulin (Ig) fold. We report the 2.8 A resolution crystal, structure of a homodimer of rod repeats 4, 5 and 6. The two chains are, arranged in an antiparallel fashion and form an elongated element, which, is shortened, however, compared to a fully extended, linear configuration, because the long axis of each Ig domain is arranged at an angle to the, long axis of the rod. Same arrangement of repeats should also be present, in the rod domain of human FLNa, much longer than Dictyostelium FLN, which, forms an extended structure able to crosslink F-actin chains over, distances of more than 1000 A.
+Dictyostelium discoideum filamin (ddFLN) is a two-chain F-actin crosslinking protein with an N-terminal actin-binding domain and a rod domain constructed from six tandem repeats of a 100 residue motif that has an immunoglobulin (Ig) fold. We report the 2.8 A resolution crystal structure of a homodimer of rod repeats 4, 5 and 6. The two chains are arranged in an antiparallel fashion and form an elongated element, which is shortened, however, compared to a fully extended, linear configuration because the long axis of each Ig domain is arranged at an angle to the long axis of the rod. Same arrangement of repeats should also be present in the rod domain of human FLNa, much longer than Dictyostelium FLN, which forms an extended structure able to crosslink F-actin chains over distances of more than 1000 A.
 ==About this Structure==
-WLH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WLH OCA].
+WLH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WLH OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Dictyostelium discoideum]]
 [[Category: Single protein]]
-[[Category: Holak, T.A.]]
+[[Category: Holak, T A.]]
 [[Category: Huber, R.]]
 [[Category: Mueller, R.]]
-[[Category: Noegel, A.A.]]
+[[Category: Noegel, A A.]]
-[[Category: Popowicz, G.M.]]
+[[Category: Popowicz, G M.]]
 [[Category: Schleicher, M.]]
 [[Category: abp-120]]
@@ Line 24: / Line 24: @@
 [[Category: rod domain]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:30:38 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:45:37 2008''