1w9p: Difference between revisions
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==Overview== | ==Overview== | ||
Family 18 chitinases play key roles in organisms ranging from bacteria to | Family 18 chitinases play key roles in organisms ranging from bacteria to man. There is a need for specific, potent inhibitors to probe the function of these chitinases in different organisms. Such molecules could also provide leads for the development of chemotherapeuticals with fungicidal, insecticidal, or anti-inflammatory potential. Recently, two natural product peptides, argifin and argadin, have been characterized, which structurally mimic chitinase-chitooligosaccharide interactions and inhibit a bacterial chitinase in the nM-mM range. Here, we show that these inhibitors also act on human and Aspergillus fumigatus chitinases. The structures of these enzymes in complex with argifin and argadin, together with mutagenesis, fluorescence, and enzymology, reveal that subtle changes in the binding site dramatically affect affinity and selectivity. The data show that it may be possible to develop specific chitinase inhibitors based on the argifin/argadin scaffolds. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Chitinase]] | [[Category: Chitinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Aalten, D | [[Category: Aalten, D M.F Van.]] | ||
[[Category: Adams, D | [[Category: Adams, D J.]] | ||
[[Category: Aerts, J | [[Category: Aerts, J M.F G.]] | ||
[[Category: Boot, R | [[Category: Boot, R G.]] | ||
[[Category: Hodkinson, M.]] | [[Category: Hodkinson, M.]] | ||
[[Category: Houston, D | [[Category: Houston, D R.]] | ||
[[Category: Omura, S.]] | [[Category: Omura, S.]] | ||
[[Category: Rao, F | [[Category: Rao, F V.]] | ||
[[Category: Shiomi, K.]] | [[Category: Shiomi, K.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: peptide inhibitors]] | [[Category: peptide inhibitors]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:41:56 2008'' | ||
Revision as of 16:42, 21 February 2008
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SPECIFICITY AND AFFINITY OF NATURAL PRODUCT CYCLOPENTAPEPTIDE INHIBITORS AGAINST ASPERGILLUS FUMIGATUS, HUMAN AND BACTERIAL CHITINASEFRA
OverviewOverview
Family 18 chitinases play key roles in organisms ranging from bacteria to man. There is a need for specific, potent inhibitors to probe the function of these chitinases in different organisms. Such molecules could also provide leads for the development of chemotherapeuticals with fungicidal, insecticidal, or anti-inflammatory potential. Recently, two natural product peptides, argifin and argadin, have been characterized, which structurally mimic chitinase-chitooligosaccharide interactions and inhibit a bacterial chitinase in the nM-mM range. Here, we show that these inhibitors also act on human and Aspergillus fumigatus chitinases. The structures of these enzymes in complex with argifin and argadin, together with mutagenesis, fluorescence, and enzymology, reveal that subtle changes in the binding site dramatically affect affinity and selectivity. The data show that it may be possible to develop specific chitinase inhibitors based on the argifin/argadin scaffolds.
About this StructureAbout this Structure
1W9P is a Single protein structure of sequence from Aspergillus fumigatus with as ligand. Active as Chitinase, with EC number 3.2.1.14 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Specificity and affinity of natural product cyclopentapeptide inhibitors against A. fumigatus, human, and bacterial chitinases., Rao FV, Houston DR, Boot RG, Aerts JM, Hodkinson M, Adams DJ, Shiomi K, Omura S, van Aalten DM, Chem Biol. 2005 Jan;12(1):65-76. PMID:15664516
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