Sandbox Reserved 703: Difference between revisions
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== '''Description''' == | == '''Description''' == | ||
[[Image:Schematic HDL particle.jpg | thumb | upright=1.5 | left | Schematic illustration of HDL particle with its associated proteins]]HPBP is a 38kDa apoliprotein, and belongs to the family of ubiquitous eukaryotic proteins named DING, an extracellular protein family including four conserved amino acids at its N-terminal end.<ref>PMID:20161715</ref><ref>PMID:12135732</ref> | [[Image:Schematic HDL particle.jpg | thumb | upright=1.5 | left | Schematic illustration of HDL particle with its associated proteins]]The human phosphate binding apoliprotein (HPBP) is a 38kDa apoliprotein, and belongs to the family of ubiquitous eukaryotic proteins named DING, an extracellular protein family including four conserved amino acids at its N-terminal end.<ref>PMID:20161715</ref><ref>PMID:12135732</ref> | ||
It is the only known transporter capable of binding phosphate ions in the human plasma. HPBP is bound to PON1, a calcium-dependent enzyme associated to HDL (High Density Lipoprotein), another lipoprotein which enables lipids like cholesterol and triglycerides to be transported from the blood to the liver. It is the place where these lipids can be removed, reducing for example the amount of arterial cholesterol. | It is the only known transporter capable of binding phosphate ions in the human plasma. HPBP is bound to PON1, a calcium-dependent enzyme associated to HDL (High Density Lipoprotein), another lipoprotein which enables lipids like cholesterol and triglycerides to be transported from the blood to the liver. It is the place where these lipids can be removed, reducing for example the amount of arterial cholesterol. | ||
HPBP is always copurified with the enzyme paraoxonase (PON1), that is why it was always ignored before 2006. The copurification is the result of a similar molecular weight, strong hydrophobic interactions, and the fact that PON1 is a glycosylated protein. The separation of the two molecules involves a hydroxyapatite chromatography with phosphate concentration gradient elution.<ref>PMID:16595195</ref><ref>PMID:19336040</ref> | HPBP is always copurified with the enzyme paraoxonase (PON1), that is why it was always ignored before 2006. The copurification is the result of a similar molecular weight, strong hydrophobic interactions, and the fact that PON1 is a glycosylated protein. The separation of the two molecules involves a hydroxyapatite chromatography with phosphate concentration gradient elution.<ref>PMID:16595195</ref><ref>PMID:19336040</ref> | ||